Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607002401_tmio GAAGGCCCTCGCTACCAAGCCTAACTGAGTTG(+ATATAT)GATCCTCAGGAACTCACAAGGAGGGACCAGA NGG 10 8 17 8 24 0 100 5 10
    240607002359_0b8b GCTGTTTTCAGACACACCAGAAGAGGGCATCG(+ATATAT)GATCCCATCACACATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240607002358_qfyz ACCACATGGTGGCTCACAACCATGTGTGATGG(+ATATAT)GATCCGATGCCCTCTTCTGGTGTGTCTGAAA NGG 10 8 17 8 24 0 100 5 10
    240607002356_na79 GGCTCACAATGCCTGTACCTCCAGCTTTGAGG(+ATATAT)GATCCTTTATTCAGGTGTACTTGGACACATG NGG 10 8 17 8 24 0 100 5 10
    240607002355_3sji GTCTACATGTGTCCAAGTACACCTGAATAAAG(+ATATAT)GATCCCTCAAAGCTGGAGGTACAGGCATTGT NGG 10 8 17 8 24 0 100 5 10
    240607002354_gcbn TGATTTCTGTGGGGGCTCAGAAGAGGGCACTG(+ATATAT)GATCCCTGGAGCTGGAGTTACAGATGGCCGT NGG 10 8 17 8 24 0 100 5 10
    240607002351_nbch AGCTCACGGCCATCTGTAACTCCAGCTCCAGG(+ATATAT)GATCCAGTGCCCTCTTCTGAGCCCCCACAGA NGG 10 8 17 8 24 0 100 5 10
    240607002350_dc7r GTTCATGGTCCTGTGTACAGTGTCAGGCATGG(+ATATAT)GTACCAGTTCTTTGAGTGCCAATTACATATT NGG 10 8 17 8 24 0 100 5 10
    240607002348_n3jy CAACCAATATGTAATTGGCACTCAAAGAACTG(+ATATAT)GTACCCATGCCTGACACTGTACACAGGACCA NGG 10 8 17 8 24 0 100 5 10
    240607002347_1ypa AGAATCCCTTGTTTGGCTAAGAGCCTGGAGAG(+ATATAT)GATCCCCTCTTCCCAAATAATTCCTCTGCCT NGG 10 8 17 8 24 0 100 5 10