Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607000840_txdr GCGTTGGTTCTGGAAGTGTGCAGAAAGGTCAG(+ATATAT)GATCCAGTCTACTCTTCTCCCAGCCACGCAA NGG 10 8 17 8 24 0 100 5 10
    240607000839_b64s GTTCTGCCAAGACCGGCAGGGTAAACAAACAG(+ATATAT)GTACCACTCTCTCCCGGGAACCCTTTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240607000837_51rz ACAACAGAAGAAAGGGTTCCCGGGAGAGAGTG(+ATATAT)GTACCTGTTTGTTTACCCTGCCGGTCTTGGC NGG 10 8 17 8 24 0 100 5 10
    240607000836_ygux CAGGAAAGGTTCCGTTTATAAACAAGAAACTG(+ATATAT)GTACCAGAAAATTGCAGAGAAGCACCAACGT NGG 10 8 17 8 24 0 100 5 10
    240607000834_5cod GGAGCACGTTGGTGCTTCTCTGCAATTTTCTG(+ATATAT)GTACCAGTTTCTTGTTTATAAACGGAACCTT NGG 10 8 17 8 24 0 100 5 10
    240607000833_iqn0 AAATAGGGAAAGAAGTCAGTGGCTGGTAGAGG(+ATATAT)GTACCACTCCCCAGATGCTGTGTAGTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240607000831_6l4k CAGGGCCTCAACTACACAGCATCTGGGGAGTG(+ATATAT)GTACCCTCTACCAGCCACTGACTTCTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240607000830_ri07 CTAAGCAGCCAATGTCGCTTCTGGGGCTCCTG(+ATATAT)GATCCTCTAGGCGGAGTCCTTTATGGTGTCC NGG 10 8 17 8 24 0 100 5 10
    240607000828_f6o4 GGGGGGGACACCATAAAGGACTCCGCCTAGAG(+ATATAT)GATCCAGGAGCCCCAGAAGCGACATTGGCTG NGG 10 8 17 8 24 0 100 5 10
    240607000827_lh1m CTTTCCTTCCACACTGTCTTGTATCATGGCTG(+ATATAT)GATCCAGTGTATTTCCAGCTAATTGTTAATG NGG 10 8 17 8 24 0 100 5 10