Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607000932_i463 AAGAGTTTGCTATTCCTACACTTTCCTGAGTG(+ATATAT)GATCCTTTCTCCCTTCCTCTGCTGATGGAAA NGG 10 8 17 8 24 0 100 5 10
    240607000930_pfvf GCCAGTTCCTAGCTGATCACAGCTTTCCTTAG(+ATATAT)GATCCAGGAAGGTCTGTGTTTTCTGAGAATA NGG 10 8 17 8 24 0 100 5 10
    240607000928_bdli AAGACTATTCTCAGAAAACACAGACCTTCCTG(+ATATAT)GATCCTAAGGAAAGCTGTGATCAGCTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240607000926_4ev3 TTTAAAAATGAAGCCCAGCTGGGTGTGGTGGG(+ATATAT)GTACCCTTCTAATCCCAGTACTTGGGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240607000924_2rrc TTCTCTGCCTCCCAAGTACTGGGATTAGAAGG(+ATATAT)GTACCCCACCACACCCAGCTGGGCTTCATTT NGG 10 8 17 8 24 0 100 5 10
    240607000922_sdxy GCTGCTTCAACCAGACTGAGCTTGGTGCCTGG(+ATATAT)GTACCCAGTGATGAGCCAAGTAAGGTAGGCA NGG 10 8 17 8 24 0 100 5 10
    240607000920_menu GCACTTGCCTACCTTACTTGGCTCATCACTGG(+ATATAT)GTACCCAGGCACCAAGCTCAGTCTGGTTGAA NGG 10 8 17 8 24 0 100 5 10
    240607000919_jwey CCTAACCCATGCGCTGCTGGTGGGTACTGAAG(+ATATAT)GTACCCACTTGGACTTGGAGGGGTAGGCTCC NGG 10 8 17 8 24 0 100 5 10
    240607000917_ev38 GTCATGGAGCCTACCCCTCCAAGTCCAAGTGG(+ATATAT)GTACCTTCAGTACCCACCAGCAGCGCATGGG NGG 10 8 17 8 24 0 100 5 10
    240607000914_j2it CAAAGTGCTCAGAGTTCCAGCCCTGGGCCCCG(+ATATAT)GTACCCATATCCATACCACTCCCTGGCTCGG NGG 10 8 17 8 24 0 100 5 10