Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607001022_wh1b CCAGCCTGTTCATGTGTTGGGGCATTGCTTAG(+ATATAT)GATCCCTGAAACATTTGCACACTGTGGTTCT NGG 10 8 17 8 24 0 100 5 10
    240607001020_qx5m CCTGTAAGTACTCCCTGAGAATGCCCATTCAG(+ATATAT)GATCCCCTGAAAGGCAAATGACAGCTATACA NGG 10 8 17 8 24 0 100 5 10
    240607001018_7nwb GGTCATGTATAGCTGTCATTTGCCTTTCAGGG(+ATATAT)GATCCTGAATGGGCATTCTCAGGGAGTACTT NGG 10 8 17 8 24 0 100 5 10
    240607001017_i6lz GATTAGTTTAACTCACAGGTACTGTCTACTTG(+ATATAT)GTACCATGCAGAAACTTTCATGCAGAATGCA NGG 10 8 17 8 24 0 100 5 10
    240607001016_jzqe CTCTTTGCATTCTGCATGAAAGTTTCTGCATG(+ATATAT)GTACCAAGTAGACAGTACCTGTGAGTTAAAC NGG 10 8 17 8 24 0 100 5 10
    240607001012_b900 TTTAATCTTTTCCCTCTGTCAGAGTACCCAGG(+ATATAT)GATCCATGAAGTGCCTGTCGCTCGAAGACTC NGG 10 8 17 8 24 0 100 5 10
    240607001011_mpb5 GCCTGGAGTCTTCGAGCGACAGGCACTTCATG(+ATATAT)GATCCCTGGGTACTCTGACAGAGGGAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607001009_ic6d CCCACTTCTCAGGAATGACCTTCCACAGCTGG(+ATATAT)GTACCCCTTAAATCTGCTATGTTCCTGCCTC NGG 10 8 17 8 24 0 100 5 10
    240607001007_it1i ACCTGGAGGCAGGAACATAGCAGATTTAAGGG(+ATATAT)GTACCCAGCTGTGGAAGGTCATTCCTGAGAA NGG 10 8 17 8 24 0 100 5 10
    240607001005_izje GTGTAACCACGGCCACCTCATTGCAGAGCAGG(+ATATAT)GATCCAGGTTAGGGAAGTATAGAGTCCCAGA NGG 10 8 17 8 24 0 100 5 10