Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607001003_idd8 TCTGTTCTGGGACTCTATACTTCCCTAACCTG(+ATATAT)GATCCCTGCTCTGCAATGAGGTGGCCGTGGT NGG 10 8 17 8 24 0 100 5 10
    240607001001_nli1 GGCACAGTGGTAGAGCCACTCTGGATTGATGG(+ATATAT)GATCCAATGGAATGACCTAGAGCTAAATGCA NGG 10 8 17 8 24 0 100 5 10
    240607001000_2l15 GGGGTTGCATTTAGCTCTAGGTCATTCCATTG(+ATATAT)GATCCCATCAATCCAGAGTGGCTCTACCACT NGG 10 8 17 8 24 0 100 5 10
    240607000957_y4j3 TTTGCTCACCTGGGCAAGAAGTCATTTCCTGG(+ATATAT)GTACCTTTCTAGCCCTGCCCATCCCAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240607000956_95ak AGAAGGAGGCTGGGATGGGCAGGGCTAGAAAG(+ATATAT)GTACCCAGGAAATGACTTCTTGCCCAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240607000955_w4qz TTCTCCCCACAGCCACAGGGAAGTTGACACTG(+ATATAT)GATCCCTCTGCTTGTCCCCATCTTTTTTGCT NGG 10 8 17 8 24 0 100 5 10
    240607000953_g55y AGGTGAGCAAAAAAGATGGGGACAAGCAGAGG(+ATATAT)GATCCAGTGTCAACTTCCCTGTGGCTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240607000952_23i3 AGCCAACAGACTTGAAAAGGCCCCTTGTCTAG(+ATATAT)GTACCCCACGAAAATAAACTTTGAAAACGTA NGG 10 8 17 8 24 0 100 5 10
    240607000950_2tb9 CTGGATACGTTTTCAAAGTTTATTTTCGTGGG(+ATATAT)GTACCTAGACAAGGGGCCTTTTCAAGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240607000949_zzwm GAGAACAGAAGCGCCTGGGGAAGAAGGGTGAG(+ATATAT)GATCCCCCAAATCAGAGTATGCCTGGGAGTG NGG 10 8 17 8 24 0 100 5 10