Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607000855_qhjo TCGTGACTTATTTCTGCCCAAAGTGTGCTCAG(+ATATAT)GATCCCCTGCCCTCAGGCTTAATAGACATAG NGG 10 8 17 8 24 0 100 5 10
    240607000854_1k9w TTACTTTGCGTGGCTGGGAGAAGAGTAGACTG(+ATATAT)GATCCTGACCTTTCTGCACACTTCCAGAACC NGG 10 8 17 8 24 0 100 5 10
    240607000853_2rue GCGTTGGTTCTGGAAGTGTGCAGAAAGGTCAG(+ATATAT)GATCCAGTCTACTCTTCTCCCAGCCACGCAA NGG 10 8 17 8 24 0 100 5 10
    240607000851_r7ju GGTCTGAAAAAGTGAGGAGACACTGGAGCATG(+ATATAT)GATCCCCAAGGTCTGAATAAGGGTTAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240607000849_kh0r ATGGACCAGCTAACCCTTATTCAGACCTTGGG(+ATATAT)GATCCATGCTCCAGTGTCTCCTCACTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240607000847_c2wh GGAGATTCATCAGAACTCAGCACACGCTGGTG(+ATATAT)GATCCACAGCACTGTCATGTCTGTTATGAAT NGG 10 8 17 8 24 0 100 5 10
    240607000846_o54d GTGTCATTCATAACAGACATGACAGTGCTGTG(+ATATAT)GATCCACCAGCGTGTGCTGAGTTCTGATGAA NGG 10 8 17 8 24 0 100 5 10
    240607000844_npey CCTAACCCATGCGCTGCTGGTGGGTACTGAAG(+ATATAT)GTACCCACTTGGACTTGGAGGGGTAGGCTCC NGG 10 8 17 8 24 0 100 5 10
    240607000843_4w7o GTCATGGAGCCTACCCCTCCAAGTCCAAGTGG(+ATATAT)GTACCTTCAGTACCCACCAGCAGCGCATGGG NGG 10 8 17 8 24 0 100 5 10
    240607000841_mwdv TTACTTTGCGTGGCTGGGAGAAGAGTAGACTG(+ATATAT)GATCCTGACCTTTCTGCACACTTCCAGAACC NGG 10 8 17 8 24 0 100 5 10