Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607001035_2a0e TTAATGCTCCAGCTTCTTGTTTCAATGTTTGG(+ATATAT)GATCCACACATGATTTGAGCTACTCCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240607001034_gpqj AGCACCCTTGTGTCTCTGGCTTCCTGTCACTG(+ATATAT)GATCCAGTACCCCTCCATGCTTGGGTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240607001033_slko TAAAACAAGGACCCAAGCATGGAGGGGTACTG(+ATATAT)GATCCAGTGACAGGAAGCCAGAGACACAAGG NGG 10 8 17 8 24 0 100 5 10
    240607001031_d0tv CTTAATATTTTCAAACTAATAGTTTATTATAG(+ATATAT)GTACCATAAATCTTTAAATAAACACTATTTT NGG 10 8 17 8 24 0 100 5 10
    240607001030_flx3 AAAATAAAATAGTGTTTATTTAAAGATTTATG(+ATATAT)GTACCTATAATAAACTATTAGTTTGAAAATA NGG 10 8 17 8 24 0 100 5 10
    240607001029_pxqb TGGAATGTCTTGGAGGCCAGAGGAGGGTGTTG(+ATATAT)GATCCCTTAGGACTGGAGTTAAAGACGAGAG NGG 10 8 17 8 24 0 100 5 10
    240607001028_jn58 CTCACCTCTCGTCTTTAACTCCAGTCCTAAGG(+ATATAT)GATCCAACACCCTCCTCTGGCCTCCAAGACA NGG 10 8 17 8 24 0 100 5 10
    240607001026_rlin ATCTAGGCTCTGGTCCTCATCTGGGGTCAAAG(+ATATAT)GATCCCTTAGGAGGGGTCAGTTTTGCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240607001025_eyzw ACAATCAGAAGCAAAACTGACCCCTCCTAAGG(+ATATAT)GATCCTTTGACCCCAGATGAGGACCAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240607001023_milc TTTAAAGAACCACAGTGTGCAAATGTTTCAGG(+ATATAT)GATCCTAAGCAATGCCCCAACACATGAACAG NGG 10 8 17 8 24 0 100 5 10