Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607000648_niy9 TATAGATAGAGGGGACCTCGTTTCAGGTGGTG(+ATATAT)GATCCATTTGGATCTGCTTCCAGAGGATTTA NGG 10 8 17 8 24 0 100 5 10
    240607000647_x8i3 CTAATATACCTCTGTTCCCAGGAGCTACTGAG(+ATATAT)GTACCCAGTGTGGTGGGGAAAAGACGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240607000645_upfa CTTAATTCACCGTCTTTTCCCCACCACACTGG(+ATATAT)GTACCTCAGTAGCTCCTGGGAACAGAGGTAT NGG 10 8 17 8 24 0 100 5 10
    240607000644_mowz TTAAACATGAAGCCTTCCCTGTGCTGGGCATG(+ATATAT)GATCCAGGCCAATGTTGGTGTCCTACCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607000642_frsn TGGGCCCCAGGTAGGACACCAACATTGGCCTG(+ATATAT)GATCCATGCCCAGCACAGGGAAGGCTTCATG NGG 10 8 17 8 24 0 100 5 10
    240607000641_vlm3 CTATATCAAGCAGAACACAGGTCATCAGCTTG(+ATATAT)GATCCATGATTTGACTTCAAGTCATTCTTTG NGG 10 8 17 8 24 0 100 5 10
    240607000640_psr0 CAGTGCAAAGAATGACTTGAAGTCAAATCATG(+ATATAT)GATCCAAGCTGATGACCTGTGTTCTGCTTGA NGG 10 8 17 8 24 0 100 5 10
    240607000637_9tbg GCTGGGGGTACTTGCCATGAAAATTCCCTTGG(+ATATAT)GATCCTGTGACCCTCTGGGGACAGAAGCACA NGG 10 8 17 8 24 0 100 5 10
    240607000636_53m5 ATGTGTGTGCTTCTGTCCCCAGAGGGTCACAG(+ATATAT)GATCCCAAGGGAATTTTCATGGCAAGTACCC NGG 10 8 17 8 24 0 100 5 10
    240607000634_op2u AATCTTGCGGAGTTCCAGAACCATACTGTGAG(+ATATAT)GTACCACAGGGTCCTGTGGCCCAGTAGTTTT NGG 10 8 17 8 24 0 100 5 10