Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607000618_bn9o TTTTAATGAGGCAGTCTCAGCATTAATAAGAG(+ATATAT)GTACCACAATTTCTGCTGGGTCTATTTCTGC NGG 10 8 17 8 24 0 100 5 10
    240607000617_mt9c TACTCTATTACCTGCAGGAAGAAACCCTAAAG(+ATATAT)GTACCCAAAGAAATAAAATTTGGCAAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240607000615_cavj AAACATCCACTTGCCAAATTTTATTTCTTTGG(+ATATAT)GTACCTTTAGGGTTTCTTCCTGCAGGTAATA NGG 10 8 17 8 24 0 100 5 10
    240607000613_uxf4 GGTCTTGAACTCAGTATGTAGTCTACATTTTG(+ATATAT)GATCCTCCTGCCCCCACCTCCTAAGTGTTGA NGG 10 8 17 8 24 0 100 5 10
    240607000612_izm8 TAATGTCAACACTTAGGAGGTGGGGGCAGGAG(+ATATAT)GATCCAAAATGTAGACTACATACTGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240607000611_6n60 CATGAATGTTTTGGACATCAAGCATCCTAATG(+ATATAT)GTACCTCCTTTGTACAATGCCAAAATGAGCC NGG 10 8 17 8 24 0 100 5 10
    240607000609_cqp8 ACCGAGGCTCATTTTGGCATTGTACAAAGGAG(+ATATAT)GTACCATTAGGATGCTTGATGTCCAAAACAT NGG 10 8 17 8 24 0 100 5 10
    240607000608_d664 CTGGAGTTGGTCATTTTTGTACACCCACAGTG(+ATATAT)GTACCTTTTACAGTCAGGTTTGGATACTTTG NGG 10 8 17 8 24 0 100 5 10
    240607000607_s2rs AGCTGCAAAGTATCCAAACCTGACTGTAAAAG(+ATATAT)GTACCACTGTGGGTGTACAAAAATGACCAAC NGG 10 8 17 8 24 0 100 5 10
    240607000604_0fap CCCCCAGTTCTCTGGCTGAGGCAACATTCCTG(+ATATAT)GTACCAGTGCCTATCACTTTGTTTCAGGACT NGG 10 8 17 8 24 0 100 5 10