Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607000533_i8qt CTCTCTCACACCTCAGCTCATTGTCTTTTGTG(+ATATAT)GTACCTGGAGTTGCTGGTTGCTATGGTAACT NGG 10 8 17 8 24 0 100 5 10
    240607000531_1xc6 TTCTGGATGTGTAATCCCTACACTTCCTAAAG(+ATATAT)GTACCCCTGGAGTGCCCCACTAATGCCCAAG NGG 10 8 17 8 24 0 100 5 10
    240607000529_s9m7 TGTGCCTTGGGCATTAGTGGGGCACTCCAGGG(+ATATAT)GTACCTTTAGGAAGTGTAGGGATTACACATC NGG 10 8 17 8 24 0 100 5 10
    240607000527_ugdf GAAGGAAGAGGGTTAAAACTTGTGATAATAAG(+ATATAT)GATCCTTCAAAACTCTGGTGCCGTCCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240607000526_925v CAGGTACTGAGGACGGCACCAGAGTTTTGAAG(+ATATAT)GATCCTTATTATCACAAGTTTTAACCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240607000525_7dwa TCACTTTCCTCCTCTGCAATTAAGAAATTGTG(+ATATAT)GTACCCTCTTCAGAAACTACACCTTTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240607000523_r8ij CCCACGAAAAAAAGGTGTAGTTTCTGAAGAGG(+ATATAT)GTACCACAATTTCTTAATTGCAGAGGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240607000521_hoov GAGGTCAACTACGAAGTGGCTGTTTCGCATTG(+ATATAT)GTACCCAGCTGTGCCTCCACTATTTGCACAC NGG 10 8 17 8 24 0 100 5 10
    240607000520_6kct AAGATGTGTGCAAATAGTGGAGGCACAGCTGG(+ATATAT)GTACCAATGCGAAACAGCCACTTCGTAGTTG NGG 10 8 17 8 24 0 100 5 10
    240607000518_k437 TCACTTTCCTCCTCTGCAATTAAGAAATTGTG(+ATATAT)GTACCCTCTTCAGAAACTACACCTTTTTTTC NGG 10 8 17 8 24 0 100 5 10