Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607000444_tbqm ACTGTGGAGAAGGACTTAAAGGAAGGTGACTG(+ATATAT)GATCCTCTCTAAGGGTCACCAGCATTTTGGT NGG 10 8 17 8 24 0 100 5 10
    240607000442_rmnp GAAGCCTTCATGAGTCTTCCACTCTCTTCTGG(+ATATAT)GTACCGACGCAGAGATGGCATGTCACAGGAT NGG 10 8 17 8 24 0 100 5 10
    240607000441_bfsv CCGGAATCCTGTGACATGCCATCTCTGCGTCG(+ATATAT)GTACCCAGAAGAGAGTGGAAGACTCATGAAG NGG 10 8 17 8 24 0 100 5 10
    240607000439_iyx0 GTTACCTCACAGACCACCACAGCCCACTCTGG(+ATATAT)GTACCATCATCCCTGTCTCTGCCATTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240607000437_93gd CTAGGGCAGGAATGGCAGAGACAGGGATGATG(+ATATAT)GTACCCAGAGTGGGCTGTGGTGGTCTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240607000436_x493 TTCTGCCAGTGTGTGTGACTATGTGAGTGTTG(+ATATAT)GATCCCCTGACACTGGAGTTACAGACAGTTG NGG 10 8 17 8 24 0 100 5 10
    240607000435_33zc GCTTACAACTGTCTGTAACTCCAGTGTCAGGG(+ATATAT)GATCCAACACTCACATAGTCACACACACTGG NGG 10 8 17 8 24 0 100 5 10
    240607000433_s3f7 GAATGTAAACAGTTTTATTCAGAAACAGATAG(+ATATAT)GTACCTGTTTGCATTACAGAATATAAAACTT NGG 10 8 17 8 24 0 100 5 10
    240607000432_4tsn AAACCAAGTTTTATATTCTGTAATGCAAACAG(+ATATAT)GTACCTATCTGTTTCTGAATAAAACTGTTTA NGG 10 8 17 8 24 0 100 5 10
    240607000431_teu2 GGAGAAGAGTGGTTTGCCTTCTGAGGTATGAG(+ATATAT)GATCCCTGGTCCAATCAGCAGGGTGAGGTTT NGG 10 8 17 8 24 0 100 5 10