Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607000341_kbsr CCATGTTGTCTTTCATAAGGAGGTGAGTTTTG(+ATATAT)GATCCACTACAGGAGGACTTCCGACGTCCAA NGG 10 8 17 8 24 0 100 5 10
    240607000340_ehga ATATGAGGCGCAGTTGCTTTCTATAGGACATG(+ATATAT)GATCCTCATACTCAGTCATTTCATGAACCCT NGG 10 8 17 8 24 0 100 5 10
    240607000339_tygd TCTCAAGGGTTCATGAAATGACTGAGTATGAG(+ATATAT)GATCCATGTCCTATAGAAAGCAACTGCGCCT NGG 10 8 17 8 24 0 100 5 10
    240607000337_rhg8 CGAATTCCTTTACTGATGACCTACCTGAGCTG(+ATATAT)GATCCTCAGCATCCACATCAAGGAAGAAAAG NGG 10 8 17 8 24 0 100 5 10
    240607000336_612m TGGTTCTTTTCTTCCTTGATGTGGATGCTGAG(+ATATAT)GATCCAGCTCAGGTAGGTCATCAGTAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240607000334_5rkm TTACTTTAGCATTTTGCATTAAGATTCAAAAG(+ATATAT)GTACCTTTTCTCTCTTTAGTGCTGTTGTAAA NGG 10 8 17 8 24 0 100 5 10
    240607000333_ou2t TAGGCTTTACAACAGCACTAAAGAGAGAAAAG(+ATATAT)GTACCTTTTGAATCTTAATGCAAAATGCTAA NGG 10 8 17 8 24 0 100 5 10
    240607000332_dgka GATGTTCTGGCTAATGTACACAAGGAGGTGTG(+ATATAT)GATCCAGCAAGTGGTTTCTTCTTTGTTTTAT NGG 10 8 17 8 24 0 100 5 10
    240607000330_jt0d CAATAATAAAACAAAGAAGAAACCACTTGCTG(+ATATAT)GATCCACACCTCCTTGTGTACATTAGCCAGA NGG 10 8 17 8 24 0 100 5 10
    240607000329_e7hd ATCATGTAATTTATCTGCTCAAAACCCTCTGG(+ATATAT)GTACCCCTATGCAGTCAAAACACTGCTTCCT NGG 10 8 17 8 24 0 100 5 10