Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607000242_md5t AGGAACGGGCTCCACGGTCTCCGCGATCTCCG(+ATATAT)GATCCCACTGCGACATTCCTGCACATGCGTG NGG 10 8 17 8 24 0 100 5 10
    240607000241_131p GCTGTCTTTGGGCACATCAGAAGAGGGCATTG(+ATATAT)GATCCCATTAATGGATGGTTGTGAGCCATCT NGG 10 8 17 8 24 0 100 5 10
    240607000239_gry5 CCACAAGATGGCTCACAACCATCCATTAATGG(+ATATAT)GATCCAATGCCCTCTTCTGATGTGCCCAAAG NGG 10 8 17 8 24 0 100 5 10
    240607000238_t6cx ATCACTCGCGAATTGCGTAAGCGGCCCTTCGG(+ATATAT)GTACCCGGGCAGAGAGAACTGCGCGGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240607000235_1aq9 GGCAGCAAGCCCGCGCAGTTCTCTCTGCCCGG(+ATATAT)GTACCCGAAGGGCCGCTTACGCAATTCGCGA NGG 10 8 17 8 24 0 100 5 10
    240607000234_5kcu AGAGGGATAGATGACTTGCGAAGGCCACACTG(+ATATAT)GTACCCTTTTCTGAGACACCCTTCCGTAGGT NGG 10 8 17 8 24 0 100 5 10
    240607000232_f5ey AAAGAACCTACGGAAGGGTGTCTCAGAAAAGG(+ATATAT)GTACCAGTGTGGCCTTCGCAAGTCATCTATC NGG 10 8 17 8 24 0 100 5 10
    240607000231_q67a GGAGACACTGTACGGTTATTATGTCCTTACTG(+ATATAT)GATCCTGAGGGTCATTTTATCTATATGCTTT NGG 10 8 17 8 24 0 100 5 10
    240607000229_ffcq TGTTGAAAGCATATAGATAAAATGACCCTCAG(+ATATAT)GATCCAGTAAGGACATAATAACCGTACAGTG NGG 10 8 17 8 24 0 100 5 10
    240607000228_c9i2 GTTACAATGGAGAAAACAGAAAAGGACTTTAG(+ATATAT)GTACCATTTATTTCCTCTGACAGTCAAAAAG NGG 10 8 17 8 24 0 100 5 10