Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607000154_dq81 TTCATAGTTCAGATGTCATAATGTTCACTAGG(+ATATAT)GATCCTCAACCAGTATATAAAATATGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240607000152_mi0j GGGCTTGACTCTGATTGATGGCCTCTGGAGAG(+ATATAT)GATCCTGCAGCTACCCAGATTCAAGGTTGGC NGG 10 8 17 8 24 0 100 5 10
    240607000151_w7ep TTGGAGCCAACCTTGAATCTGGGTAGCTGCAG(+ATATAT)GATCCTCTCCAGAGGCCATCAATCAGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240607000150_5v9m ACAGGGTAATTCATTAGCAAGACATTTCATTG(+ATATAT)GTACCGGGTTCAGGTTGTCTGGGGAATAATG NGG 10 8 17 8 24 0 100 5 10
    240607000148_f2tv CCTCCCATTATTCCCCAGACAACCTGAACCCG(+ATATAT)GTACCAATGAAATGTCTTGCTAATGAATTAC NGG 10 8 17 8 24 0 100 5 10
    240607000145_22ik CCCCCGGGTCCAGCTCTGCTCCTGTGCCTGGG(+ATATAT)GATCCGGAGGCTCCTGGCTGTCTTGAAATGG NGG 10 8 17 8 24 0 100 5 10
    240607000143_7jf7 AAAGTCCATTTCAAGACAGCCAGGAGCCTCCG(+ATATAT)GATCCCCAGGCACAGGAGCAGAGCTGGACCC NGG 10 8 17 8 24 0 100 5 10
    240607000140_xo3o AGGTGTGGCTGTAAGCTTCCAGGCTTCCCCTG(+ATATAT)GTACCCGGCATGCCAGGGTAGCCTGCACCTG NGG 10 8 17 8 24 0 100 5 10
    240607000138_ibma TCCTGCAGGTGCAGGCTACCCTGGCATGCCGG(+ATATAT)GTACCAGGGGAAGCCTGGAAGCTTACAGCCA NGG 10 8 17 8 24 0 100 5 10
    240607000136_b684 GCACCCTGTGAGTGTAGAATGTTTTGTAAGTG(+ATATAT)GATCCTCTTTCCTTGAAATCTGTAAGCTCAA NGG 10 8 17 8 24 0 100 5 10