Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607000135_0a2o GGACATTGAGCTTACAGATTTCAAGGAAAGAG(+ATATAT)GATCCACTTACAAAACATTCTACACTCACAG NGG 10 8 17 8 24 0 100 5 10
    240607000133_m0fq ATCAAGTTGGTCCTACAGATGGAACATCCATG(+ATATAT)GATCCGCAATTCAAACATGTATGAAAAATAA NGG 10 8 17 8 24 0 100 5 10
    240607000132_21el ATATTTTATTTTTCATACATGTTTGAATTGCG(+ATATAT)GATCCATGGATGTTCCATCTGTAGGACCAAC NGG 10 8 17 8 24 0 100 5 10
    240607000130_odwe GGACAGACAGGAAGTACACATGAGACACCAAG(+ATATAT)GATCCAAAACAACAGGCAGGGCTGGGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240607000128_j90h GCTAATGCAGCCCAGCCCTGCCTGTTGTTTTG(+ATATAT)GATCCTTGGTGTCTCATGTGTACTTCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240607000127_4nxk AAGAGATTTGAAACACATTTGAGCTGTAAGTG(+ATATAT)GTACCTGAGGACCAGAAGATGGCCATGGGCG NGG 10 8 17 8 24 0 100 5 10
    240607000125_ed7k GAGGCCGCCCATGGCCATCTTCTGGTCCTCAG(+ATATAT)GTACCACTTACAGCTCAAATGTGTTTCAAAT NGG 10 8 17 8 24 0 100 5 10
    240607000123_0676 GGCCTAGTGTATCTATTATCTAGTTCTTTCGG(+ATATAT)GTACCAGGGTTCTCCCCCTATCCTGTGTTCA NGG 10 8 17 8 24 0 100 5 10
    240607000121_0mfx TGCTCTGAACACAGGATAGGGGGAGAACCCTG(+ATATAT)GTACCCGAAAGAACTAGATAATAGATACACT NGG 10 8 17 8 24 0 100 5 10
    240607000119_tzjc CTCCAGCTTCCACCTCAGAGAAAACACCAAAG(+ATATAT)GTACCAAGAGCTAAGCCCAGGGGCCAACCAA NGG 10 8 17 8 24 0 100 5 10