Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607000118_2yma TGTTCTTGGTTGGCCCCTGGGCTTAGCTCTTG(+ATATAT)GTACCTTTGGTGTTTTCTCTGAGGTGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240607000116_ncyj TAACTCCCAGAATCAAAAGGGCCCGAGGGAGG(+ATATAT)GTACCTGTGAACGGTTCCCGCGGGTGGGTCT NGG 10 8 17 8 24 0 100 5 10
    240607000114_y64y TTCTTAGACCCACCCGCGGGAACCGTTCACAG(+ATATAT)GTACCCTCCCTCGGGCCCTTTTGATTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607000113_fo50 CAGGCTGGCTGCGAAAGGAGGCTACACATATG(+ATATAT)GATCCACCTGACTGTAATCAGTGAATCTCAA NGG 10 8 17 8 24 0 100 5 10
    240607000111_wj0u AGAAGTTGAGATTCACTGATTACAGTCAGGTG(+ATATAT)GATCCATATGTGTAGCCTCCTTTCGCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240607000110_n88w GCTGGGGAGGCCAGTGTATCTGCTGGAGTCTG(+ATATAT)GATCCTGACATTTACCTATTTCTTATTGGCA NGG 10 8 17 8 24 0 100 5 10
    240607000108_y87q AGGATTGCCAATAAGAAATAGGTAAATGTCAG(+ATATAT)GATCCAGACTCCAGCAGATACACTGGCCTCC NGG 10 8 17 8 24 0 100 5 10
    240607000106_08vf AGTCCCAACACCTATGCGTGTTCCCAACTCCG(+ATATAT)GATCCTGTTAATACTATGGTTAAAAGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240607000105_qatc TATGGAACCACTTTTAACCATAGTATTAACAG(+ATATAT)GATCCGGAGTTGGGAACACGCATAGGTGTTG NGG 10 8 17 8 24 0 100 5 10
    240607000103_fxvj TCTGGGCAGTAAGGACCCTATCACTGAGCCAG(+ATATAT)GTACCTTGTCCTCTTCTTATTTTGAAGCATC NGG 10 8 17 8 24 0 100 5 10