Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607000010_sgt4 TATGTTTAACACTTAGTGCTTTAAAAGCAGTG(+ATATAT)GTACCCAATACGATGGGAATCCGAGTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240607000009_8m3j GAGCATCCTCTGTCCTGCCTCGTGCCCAGATG(+ATATAT)GTACCTCTGGAGAAGGCATGCGGCTGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240607000007_p1y5 CAGATGAGGCCAGCCGCATGCCTTCTCCAGAG(+ATATAT)GTACCATCTGGGCACGAGGCAGGACAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240607000004_ac11 GGCCGCTCTGTTCCCCCTGACCTAGCCTCGGG(+ATATAT)GTACCCTGTGGCCATTTGTGGTGACTTTGCC NGG 10 8 17 8 24 0 100 5 10
    240607000003_abpx GGAGAGGCAAAGTCACCACAAATGGCCACAGG(+ATATAT)GTACCCCGAGGCTAGGTCAGGGGGAACAGAG NGG 10 8 17 8 24 0 100 5 10
    240607000001_tnu3 ACACCAGGCACACAGAGAGAAGTTCATGCCAG(+ATATAT)GTACCAAGGGAAGATGTGAGACAGAGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606235958_g16u AGAGGGCTGGCTCTGTCTCACATCTTCCCTTG(+ATATAT)GTACCTGGCATGAACTTCTCTCTGTGTGCCT NGG 10 8 17 8 24 0 100 5 10
    240606235956_0fo9 CAGCCTAAGAATCAAGCTAAGGTATTATCAGG(+ATATAT)GATCCCAAATGCCCCTCCCACCCAGGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606235954_pj1m TAACGTCCTTCCTGGGTGGGAGGGGCATTTGG(+ATATAT)GATCCCTGATAATACCTTAGCTTGATTCTTA NGG 10 8 17 8 24 0 100 5 10
    240606235952_r07a GGGCTCAGGCTCTTGTTGTGGGCGCTGCCTGG(+ATATAT)GATCCATTCCTGAGGAGCTGGGCTAGATAGA NGG 10 8 17 8 24 0 100 5 10