Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606235914_7ynx AGGGTGGCACAGCAGCCTGGCGTCAGTGACTG(+ATATAT)GTACCCCTTCTCCTGTGTCCAGTGCAGCCAT NGG 10 8 17 8 24 0 100 5 10
    240606235913_zicc ACAAGAAAGCCGCTCTCGTCTGCAGGACACGG(+ATATAT)GATCCTGCGTGGCTCACAGCGGCAGCTGCCA NGG 10 8 17 8 24 0 100 5 10
    240606235911_tj9x AGAAGTGGCAGCTGCCGCTGTGAGCCACGCAG(+ATATAT)GATCCCGTGTCCTGCAGACGAGAGCGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240606235910_wnjd CCAGTTTTTCTCAGATTTAGGGAGAATACATG(+ATATAT)GTACCTTTTACCTACCCATACTAGAAACTAA NGG 10 8 17 8 24 0 100 5 10
    240606235909_oc7r TGTAGTTAGTTTCTAGTATGGGTAGGTAAAAG(+ATATAT)GTACCATGTATTCTCCCTAAATCTGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606235907_3d2i AAGTGGGGAGGAATGGTACTATTGGCATGGGG(+ATATAT)GTACCTGGTATTGAAAATGGACACATAAACT NGG 10 8 17 8 24 0 100 5 10
    240606235905_1q6x CAGCTAGTTTATGTGTCCATTTTCAATACCAG(+ATATAT)GTACCCCCATGCCAATAGTACCATTCCTCCC NGG 10 8 17 8 24 0 100 5 10
    240606235903_kwcd GGGGATCTGATGTCCTCTTCTGGCCTCTGTTG(+ATATAT)GTACCTGCACACACATGGTGTACACATACAC NGG 10 8 17 8 24 0 100 5 10
    240606235902_uxsq TATTTGTGTATGTGTACACCATGTGTGTGCAG(+ATATAT)GTACCAACAGAGGCCAGAAGAGGACATCAGA NGG 10 8 17 8 24 0 100 5 10
    240606235901_3dg8 GATTCTAGTCTGACTTGGAACAGAGGTCACAG(+ATATAT)GTACCAACCTGATGAGACCTGCTGCTATTGT NGG 10 8 17 8 24 0 100 5 10