Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606235843_gxss AGACTTTTCATTGGAAAAGCCTAGCCTCTGGG(+ATATAT)GTACCCCTTCTCGTGTGTATTGTGGAAAGCG NGG 10 8 17 8 24 0 100 5 10
    240606235841_5sus GGAAGCCAGGCAGGACAGGTCCATGGGGCCTG(+ATATAT)GTACCCACTGGCCAAAAATAACAGTGTGATT NGG 10 8 17 8 24 0 100 5 10
    240606235840_zk6x AAAAAAATCACACTGTTATTTTTGGCCAGTGG(+ATATAT)GTACCAGGCCCCATGGACCTGTCCTGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606235837_rd7c CAAAGCAGCAGAGAATAGAGGACGGAGCCCAG(+ATATAT)GTACCTCCTCTTCCTCCTCCTCACCGTCGCC NGG 10 8 17 8 24 0 100 5 10
    240606235835_zr17 ATGGAGGCGACGGTGAGGAGGAGGAAGAGGAG(+ATATAT)GTACCTGGGCTCCGTCCTCTATTCTCTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606235833_5xsp TGGGAAATAGACCCTGCAGTTACCTAGAATGG(+ATATAT)GTACCAGAGAGATGATGCTGGAAGCGCCGAC NGG 10 8 17 8 24 0 100 5 10
    240606235832_glwk ATACTGTCGGCGCTTCCAGCATCATCTCTCTG(+ATATAT)GTACCCATTCTAGGTAACTGCAGGGTCTATT NGG 10 8 17 8 24 0 100 5 10
    240606235831_aqol AAAACCAATTTACTGAAAAACTGCACAAGAAG(+ATATAT)GTACCGTGAATGTAATTTCCTAGCAGGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606235830_gj97 GCAGAGTGGCCTGCTAGGAAATTACATTCACG(+ATATAT)GTACCTTCTTGTGCAGTTTTTCAGTAAATTG NGG 10 8 17 8 24 0 100 5 10
    240606235828_fvoy TTTTCTTTGGAGTCTGGATTTGGTGCCCGTGG(+ATATAT)GATCCTGGAGGGCCCCACCCCCATCCTGAAG NGG 10 8 17 8 24 0 100 5 10