Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606235933_670v CTGTTTACCACCCCAGAAACCCAGGCAAGCTG(+ATATAT)GATCCCCAGCTAGGTTCCACGCGCCCTGCGC NGG 10 8 17 8 24 0 100 5 10
    240606235932_4q0l CCGAGTCCAGGTGCCCATGGAAGAAAGCATTG(+ATATAT)GATCCCCCTGGAGTTGAAGTCACCTAATGTA NGG 10 8 17 8 24 0 100 5 10
    240606235930_ix0j ACATCTACATTAGGTGACTTCAACTCCAGGGG(+ATATAT)GATCCAATGCTTTCTTCCATGGGCACCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606235928_huwd AAAAGACCCCAGAAGCACGTGGGTGAGGCCTG(+ATATAT)GATCCAGGCACCTGTGGGAGGTGAGCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606235926_a0i5 ACATGAGGAGGCTCACCTCCCACAGGTGCCTG(+ATATAT)GATCCAGGCCTCACCCACGTGCTTCTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240606235924_g2kn TGGTCAAGCTCAGCAGCCCCTGGGTCCCCTTG(+ATATAT)GTACCAGTCAAAGGTGGAGCAATCCCGAACT NGG 10 8 17 8 24 0 100 5 10
    240606235922_799l CCCAAAGTTCGGGATTGCTCCACCTTTGACTG(+ATATAT)GTACCAAGGGGACCCAGGGGCTGCTGAGCTT NGG 10 8 17 8 24 0 100 5 10
    240606235920_ccnq GGCCACCCCAACAGATGGGCTCCCTTCTATGG(+ATATAT)GATCCCCAAAGGGCAGGTCCTGGCCGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606235918_t7y8 AGCTGTCCAGCGGCCAGGACCTGCCCTTTGGG(+ATATAT)GATCCCATAGAAGGGAGCCCATCTGTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606235916_g0kb ATGCCATGGCTGCACTGGACACAGGAGAAGGG(+ATATAT)GTACCAGTCACTGACGCCAGGCTGCTGTGCC NGG 10 8 17 8 24 0 100 5 10