Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607000047_6w0h CTAAGCAGCGGGGCTCTAGGGCACCTGAACCG(+ATATAT)GATCCGTGATGAACTAATAGGCCTTGCCCGC NGG 10 8 17 8 24 0 100 5 10
    240607000044_hi57 CCCTGACAAAACTCATTCTACCCAGTACCCAG(+ATATAT)GATCCTGGGCACACGCACATCTATATGGAAA NGG 10 8 17 8 24 0 100 5 10
    240607000042_d1js AACCATTTCCATATAGATGTGCGTGTGCCCAG(+ATATAT)GATCCTGGGTACTGGGTAGAATGAGTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240607000040_ebgv GAGGATTGACTCCTCAGAGAAGGAGCCTATGG(+ATATAT)GTACCTGGGGCCACGTACAGCAGGGCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240607000037_oc1x CTGCCGCTGAGCCCTGCTGTACGTGGCCCCAG(+ATATAT)GTACCCATAGGCTCCTTCTCTGAGGAGTCAA NGG 10 8 17 8 24 0 100 5 10
    240607000036_ztf3 CTTGGAAAGATGGGCCACATGCTGCATGACAG(+ATATAT)GATCCTCCAAGTACCTTAACTGTTAGAGTAT NGG 10 8 17 8 24 0 100 5 10
    240607000034_w6nf CACGGATACTCTAACAGTTAAGGTACTTGGAG(+ATATAT)GATCCTGTCATGCAGCATGTGGCCCATCTTT NGG 10 8 17 8 24 0 100 5 10
    240607000032_vzgo AGTGACCCTCCCAACACCTCTGTGCCAGCAGG(+ATATAT)GATCCCCAAGGCAGCTACCACCTTAACATCT NGG 10 8 17 8 24 0 100 5 10
    240607000030_b2us GTCCTAGATGTTAAGGTGGTAGCTGCCTTGGG(+ATATAT)GATCCCTGCTGGCACAGAGGTGTTGGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240607000029_3zk9 GACCTCTTCCTGTGAATGTGACCCATTCATTG(+ATATAT)GTACCTGAAGTTATTAGTAGTATCCAATGCC NGG 10 8 17 8 24 0 100 5 10