Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606235756_rkmx TACCCGCCAGCTGGTCAGGAGTCTGCACGTTG(+ATATAT)GTACCCCTTTTCCCTGAACTGGGAGTAATCG NGG 10 8 17 8 24 0 100 5 10
    240606235754_mypj CTGCCTCCAGAACACTGCAGGATTCTCCACCG(+ATATAT)GTACCTGCTGGTTTCCCATAGGCCCAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240606235753_kols CTGAGCAGCCTGGGCCTATGGGAAACCAGCAG(+ATATAT)GTACCGGTGGAGAATCCTGCAGTGTTCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606235751_9lqd GAAGACAGAGCCCACACAGTAGGAAATTCCAG(+ATATAT)GTACCAACACTGTAAAGTATGTGTACTGAGA NGG 10 8 17 8 24 0 100 5 10
    240606235750_7qdu TCTCCTCTCAGTACACATACTTTACAGTGTTG(+ATATAT)GTACCTGGAATTTCCTACTGTGTGGGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240606235749_rcvt TCTGTGCAGCAAAATGCTGGGTACTGTTTATG(+ATATAT)GATCCAGTCTGTTAGTCTATGTCTCTTTATT NGG 10 8 17 8 24 0 100 5 10
    240606235748_kmz2 TCCACAATAAAGAGACATAGACTAACAGACTG(+ATATAT)GATCCATAAACAGTACCCAGCATTTTGCTGC NGG 10 8 17 8 24 0 100 5 10
    240606235746_cdu5 AGACGAATGCCGAAGCCTGGCATCAGGGACGG(+ATATAT)GATCCCATCCTCTGGAGTAGGCAATAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240606235744_utgt GTTTTCCACCTATTGCCTACTCCAGAGGATGG(+ATATAT)GATCCCGTCCCTGATGCCAGGCTTCGGCATT NGG 10 8 17 8 24 0 100 5 10
    240606235742_p2s2 AGGGGTTCCAGATGCTCAGCAGACACTCCTGG(+ATATAT)GATCCAAGGAAGCACTTATGCCAACCAGGAA NGG 10 8 17 8 24 0 100 5 10