Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606235650_56fp ACTCAAGCAGGGCAAGGACTCAAGCAGGGCAG(+ATATAT)GATCCTGGAGGCAGGAGCTGATGCAGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240606235649_98s0 GACACAGGGTGCTAAGTCTCACTGAACACTGG(+ATATAT)GTACCCTAATAAGACTCAAACAGGCTCCATC NGG 10 8 17 8 24 0 100 5 10
    240606235647_f628 GAGGGGATGGAGCCTGTTTGAGTCTTATTAGG(+ATATAT)GTACCCAGTGTTCAGTGAGACTTAGCACCCT NGG 10 8 17 8 24 0 100 5 10
    240606235645_3wpc GCTCTAGGCTGGTGGGAGAGGGGGTGTCCTGG(+ATATAT)GATCCCCTCCAGGGACAAGTGCTCTGGGGGG NGG 10 8 17 8 24 0 100 5 10
    240606235643_w9mx AACTTCCCCCCAGAGCACTTGTCCCTGGAGGG(+ATATAT)GATCCCAGGACACCCCCTCTCCCACCAGCCT NGG 10 8 17 8 24 0 100 5 10
    240606235642_8f0g CGTTGGATACATTGCCACAGTAACTGAAGAAG(+ATATAT)GTACCCGGAGCCCAGTGTGAATCGTTTATAT NGG 10 8 17 8 24 0 100 5 10
    240606235640_8cxu TTGGAATATAAACGATTCACACTGGGCTCCGG(+ATATAT)GTACCTTCTTCAGTTACTGTGGCAATGTATC NGG 10 8 17 8 24 0 100 5 10
    240606235638_s1ol CCAGAGCCAAACCCTGTAGGTGCCCAGTGTGG(+ATATAT)GATCCAGTATCTCATTTGGTGGTATCTTTAT NGG 10 8 17 8 24 0 100 5 10
    240606235637_rg36 CACCAATAAAGATACCACCAAATGAGATACTG(+ATATAT)GATCCCACACTGGGCACCTACAGGGTTTGGC NGG 10 8 17 8 24 0 100 5 10
    240606235635_rs08 TTGCACTGCAAACTTGAGTTCTTTACTGAAGG(+ATATAT)GTACCAATGCATCCATCCATAAAGGTCCAGA NGG 10 8 17 8 24 0 100 5 10