Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606235601_sr6v TTGAGTCTCTGACTCAGCAGCGAGGCAGAGTG(+ATATAT)GATCCGAAAGAGAACTGCTCAGACATGAGAG NGG 10 8 17 8 24 0 100 5 10
    240606235600_fs9i AAAAAAAAAATAAATGTTTTCAAGTGTGTTGG(+ATATAT)GTACCTAAGGGTCTTTGACAACCCCAGTACC NGG 10 8 17 8 24 0 100 5 10
    240606235558_0vlc GCAAAGGTACTGGGGTTGTCAAAGACCCTTAG(+ATATAT)GTACCCAACACACTTGAAAACATTTATTTTT NGG 10 8 17 8 24 0 100 5 10
    240606235556_ybam GTATGCTGTGACCTACTTTCAGGTGTTAAACG(+ATATAT)GATCCTGCGTAGCTTCCCTGAGCACTCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606235555_jgyy AGAGGCAAAGAGTGCTCAGGGAAGCTACGCAG(+ATATAT)GATCCGTTTAACACCTGAAAGTAGGTCACAG NGG 10 8 17 8 24 0 100 5 10
    240606235554_wpog CCCTCCAGGCTGCTGATACCCACTTAGAGAGG(+ATATAT)GATCCCTCCAGACACCCCCCCACTCTGACAC NGG 10 8 17 8 24 0 100 5 10
    240606235552_950l ACAAAGTGTCAGAGTGGGGGGGTGTCTGGAGG(+ATATAT)GATCCCTCTCTAAGTGGGTATCAGCAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240606235550_0apd GAGAAGAGAAAGAGATCTCGGGACGTCTGTGG(+ATATAT)GTACCTTCATTCAGAAATACAATGGACAAGG NGG 10 8 17 8 24 0 100 5 10
    240606235549_6zvg CCCACCCTTGTCCATTGTATTTCTGAATGAAG(+ATATAT)GTACCCACAGACGTCCCGAGATCTCTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240606235547_ay3b CTGCGCCCTGCACAGCAGACAATGTGTTCTGG(+ATATAT)GTACCCCCAGCCTTTGCCATCTCAGGTCAAG NGG 10 8 17 8 24 0 100 5 10