Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606235458_xw87 ACCTTTCATCTGGCCGAGCCAGGGGCAAAGGG(+ATATAT)GATCCTAAGCAAATGCCTTCCGTGGACAACA NGG 10 8 17 8 24 0 100 5 10
    240606235457_lu1g TTACATGATAAGAAAGCTTGACACAAGCTGAG(+ATATAT)GTACCAAGAGGCTCCTGCCACCTGGTCCACG NGG 10 8 17 8 24 0 100 5 10
    240606235455_mmqp TCACCCGTGGACCAGGTGGCAGGAGCCTCTTG(+ATATAT)GTACCTCAGCTTGTGTCAAGCTTTCTTATCA NGG 10 8 17 8 24 0 100 5 10
    240606235454_t52i ACCCTAAACTCAAGTCAAGGTTAATGCATGCG(+ATATAT)GATCCCGTGACGGGGCTCAGTGAGGCCCTGC NGG 10 8 17 8 24 0 100 5 10
    240606235453_prcn CCCTGGCAGGGCCTCACTGAGCCCCGTCACGG(+ATATAT)GATCCGCATGCATTAACCTTGACTTGAGTTT NGG 10 8 17 8 24 0 100 5 10
    240606235451_btk0 GACTCTGGGCACGCCCTTGGCTCCCTCGGAGG(+ATATAT)GTACCGGCTCCTCCAGAGACTAGGTTTATTT NGG 10 8 17 8 24 0 100 5 10
    240606235449_ic7e GCAAAAAATAAACCTAGTCTCTGGAGGAGCCG(+ATATAT)GTACCCTCCGAGGGAGCCAAGGGCGTGCCCA NGG 10 8 17 8 24 0 100 5 10
    240606235448_37cz CTAAAGGGTGGTGACTGGCTTCAGGGGGTGTG(+ATATAT)GTACCATCTGTGCCTGCTGCCAAGACGCAGG NGG 10 8 17 8 24 0 100 5 10
    240606235446_7l2r TTGATCCTGCGTCTTGGCAGCAGGCACAGATG(+ATATAT)GTACCACACCCCCTGAAGCCAGTCACCACCC NGG 10 8 17 8 24 0 100 5 10
    240606235445_c669 GGAAGTATAGCCAAGATAGGCCCTGGGTCATG(+ATATAT)GTACCTGCTCACTGGAGGTCTGCAAAGTAGG NGG 10 8 17 8 24 0 100 5 10