Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606235429_my71 CACTCCCTATAGTCATGATGGTCCGGATGCGG(+ATATAT)GTACCGTGCTTCACGGACTGCAGAGCAGCAC NGG 10 8 17 8 24 0 100 5 10
    240606235427_w4if GGTACAGCTGGATGCTGCAGAGGAGGGACTGG(+ATATAT)GTACCATGATGGCTCCCCTAGGGAGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606235426_pmhz TCTCCTGCTGCCTCCCTAGGGGAGCCATCATG(+ATATAT)GTACCCAGTCCCTCCTCTGCAGCATCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240606235424_un0n GGTACAGCTGGATGCTGCAGAGGAGGGACTGG(+ATATAT)GTACCATGATGGCTCCCCTAGGGAGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606235423_zfw1 TCTCCTGCTGCCTCCCTAGGGGAGCCATCATG(+ATATAT)GTACCCAGTCCCTCCTCTGCAGCATCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240606235421_vt0u GTGTGTGGTTTCCATCCCAAGGATGCCTGGTG(+ATATAT)GTACCACGGCTCCCAGGAAGAAGGTTGGAGA NGG 10 8 17 8 24 0 100 5 10
    240606235420_23v4 CACACTCTCCAACCTTCTTCCTGGGAGCCGTG(+ATATAT)GTACCACCAGGCATCCTTGGGATGGAAACCA NGG 10 8 17 8 24 0 100 5 10
    240606235418_2xey GCCACCCAGACTGTGGTAGCTTGGCATGAATG(+ATATAT)GTACCGGCTGTTCTTATGAGTGTGAATGTGT NGG 10 8 17 8 24 0 100 5 10
    240606235417_iuk0 CACATACACATTCACACTCATAAGAACAGCCG(+ATATAT)GTACCATTCATGCCAAGCTACCACAGTCTGG NGG 10 8 17 8 24 0 100 5 10
    240606235415_5r3z CCTCGCCCCTGTGGGAAGGCAGGGCCAAAAAG(+ATATAT)GTACCACATTCTCACTCAAAAAAAAACAAAC NGG 10 8 17 8 24 0 100 5 10