Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606235357_jxfo TGTGACAATGTGCTATAGAGAGTAGGATCTGG(+ATATAT)GATCCTGGCTGTGAATGCTGAACTGATCTCA NGG 10 8 17 8 24 0 100 5 10
    240606235355_80v7 CTGGGGGACCATAGATATGATGAAAATAAAAG(+ATATAT)GTACCTGGAATTTTTGACACATGTAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240606235354_mger TGTTTCAAAGTTACATGTGTCAAAAATTCCAG(+ATATAT)GTACCTTTTATTTTCATCATATCTATGGTCC NGG 10 8 17 8 24 0 100 5 10
    240606235352_d1la ACTGCTAGGCTTTTTGATGCAAGTTGGATTGG(+ATATAT)GATCCTTCCAGGTCTCTTCTTATACATACAC NGG 10 8 17 8 24 0 100 5 10
    240606235351_sofg CTTGTGTGTATGTATAAGAAGAGACCTGGAAG(+ATATAT)GATCCCAATCCAACTTGCATCAAAAAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240606235349_5ekm CTGGGGGCCCATAGGTATGATGAAAATAAAAG(+ATATAT)GTACCTGGAATTTTTGACACATGTAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240606235347_t9f9 TGTTTCAAAGTTACATGTGTCAAAAATTCCAG(+ATATAT)GTACCTTTTATTTTCATCATACCTATGGGCC NGG 10 8 17 8 24 0 100 5 10
    240606235346_zkkl ACTGCTAGGCTTTTTGATGCAAGTTGGATTGG(+ATATAT)GATCCTTCCAGGTCTCTTCTTACACATACAC NGG 10 8 17 8 24 0 100 5 10
    240606235344_z5tw CTTGTGTGTATGTGTAAGAAGAGACCTGGAAG(+ATATAT)GATCCCAATCCAACTTGCATCAAAAAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240606235343_h7za CTGTGGAAGAGTTTCTATGTGAGATGAGGATG(+ATATAT)GATCCCTCAGAGTACACACCCATCTCATGGC NGG 10 8 17 8 24 0 100 5 10