Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606235341_0vm6 AGCAAGCCATGAGATGGGTGTGTACTCTGAGG(+ATATAT)GATCCATCCTCATCTCACATAGAAACTCTTC NGG 10 8 17 8 24 0 100 5 10
    240606235339_texj ACTGTTACACGTATTGATGTTGGGTAGCTTGG(+ATATAT)GATCCTTCTAGATCTCTGATGCAAGAAACCC NGG 10 8 17 8 24 0 100 5 10
    240606235338_g9j0 AGTCTGGGTTTCTTGCATCAGAGATCTAGAAG(+ATATAT)GATCCCAAGCTACCCAACATCAATACGTGTA NGG 10 8 17 8 24 0 100 5 10
    240606235337_1uz7 GTACAGTGGATCTGAGCGGCAGGGGATTATTG(+ATATAT)GTACCATCTGCTCTTCGGGCCCAACGGGGTA NGG 10 8 17 8 24 0 100 5 10
    240606235335_mws4 GGGACTACCCCGTTGGGCCCGAAGAGCAGATG(+ATATAT)GTACCAATAATCCCCTGCCGCTCAGATCCAC NGG 10 8 17 8 24 0 100 5 10
    240606235332_cdxp TGCCTGGTTCTAGGCCCCTTCCTCTACCCCAG(+ATATAT)GTACCCTCAGCCTAGGGGAGCAGGCTCAGGT NGG 10 8 17 8 24 0 100 5 10
    240606235331_1ck8 CTTCCACCTGAGCCTGCTCCCCTAGGCTGAGG(+ATATAT)GTACCTGGGGTAGAGGAAGGGGCCTAGAACC NGG 10 8 17 8 24 0 100 5 10
    240606235329_niys CCCCCGGGAACCCCCACCCCCACCCCCGGCAG(+ATATAT)GATCCCTCCATGCAGGAGAAAGCCAACCAGA NGG 10 8 17 8 24 0 100 5 10
    240606235327_p268 GGGCCTCTGGTTGGCTTTCTCCTGCATGGAGG(+ATATAT)GATCCTGCCGGGGGTGGGGGTGGGGGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606235324_em9h TGTCGCCCAGGGCTGTAAGCTGAGACCCCTGG(+ATATAT)GATCCCTGTCTAAGCTAGATGCCCCTTCCCT NGG 10 8 17 8 24 0 100 5 10