Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606235545_ewly TTTACCTTGACCTGAGATGGCAAAGGCTGGGG(+ATATAT)GTACCCAGAACACATTGTCTGCTGTGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240606235544_ufgx CACCAGGTTGTGGGAAACTGGTGTAACTGTAG(+ATATAT)GATCCATCCTAAGCAAGAACGGGAGGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240606235542_zrcx TTTTTCTTGGCCTCCCGTTCTTGCTTAGGATG(+ATATAT)GATCCTACAGTTACACCAGTTTCCCACAACC NGG 10 8 17 8 24 0 100 5 10
    240606235541_mkeu ATTTTTTTTGTTAGTTTCATCCAGATGTTCAG(+ATATAT)GATCCAACAAATGTTATTTTCTAAACCCGCC NGG 10 8 17 8 24 0 100 5 10
    240606235540_7i9g AGGAGGGCGGGTTTAGAAAATAACATTTGTTG(+ATATAT)GATCCTGAACATCTGGATGAAACTAACAAAA NGG 10 8 17 8 24 0 100 5 10
    240606235538_jqen GAAGGAGCCATGTGCCGGAGCAGCCGGAGGTG(+ATATAT)GATCCCCACACCAGTGGCTTAGCAGGAAGGG NGG 10 8 17 8 24 0 100 5 10
    240606235536_0ngt TCCCTCCCTTCCTGCTAAGCCACTGGTGTGGG(+ATATAT)GATCCACCTCCGGCTGCTCCGGCACATGGCT NGG 10 8 17 8 24 0 100 5 10
    240606235535_qv2r AAACCCAACAGTATCCTGAAGAAGACAGTGTG(+ATATAT)GATCCATGTTTAAATAAGGAGATGGGACAGG NGG 10 8 17 8 24 0 100 5 10
    240606235533_rytt ACCACCCTGTCCCATCTCCTTATTTAAACATG(+ATATAT)GATCCACACTGTCTTCTTCAGGATACTGTTG NGG 10 8 17 8 24 0 100 5 10
    240606235532_w2pw TGCAAAGACAACAGTGTTTTAAAGTGGATTGG(+ATATAT)GTACCAGGCTCACAAGCCTAGGTGATGTGCT NGG 10 8 17 8 24 0 100 5 10