Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606235634_6ppv GGAGATCTGGACCTTTATGGATGGATGCATTG(+ATATAT)GTACCCTTCAGTAAAGAACTCAAGTTTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606235633_r6fi AGCATGACGGTATGCATCTATCGTGAGCATAG(+ATATAT)GATCCTAAGAGAAGGTTTAGTTAGCTGGTAC NGG 10 8 17 8 24 0 100 5 10
    240606235631_w3vj CAGGTGTACCAGCTAACTAAACCTTCTCTTAG(+ATATAT)GATCCTATGCTCACGATAGATGCATACCGTC NGG 10 8 17 8 24 0 100 5 10
    240606235630_kwas TGCTGATGAAATCTGAACCATGCATCTTTTGG(+ATATAT)GATCCACCTCCTTCAGTTTGGGGCAGATCTT NGG 10 8 17 8 24 0 100 5 10
    240606235628_o1rw TCTGCAAGATCTGCCCCAAACTGAAGGAGGTG(+ATATAT)GATCCCAAAAGATGCATGGTTCAGATTTCAT NGG 10 8 17 8 24 0 100 5 10
    240606235626_uoex CACTTTCCATCTTGTGTTAAACATTTACCCAG(+ATATAT)GTACCTGGGTATTTTTGTTGTCAATTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240606235623_zerp TGGAAACCCCAATTGACAACAAAAATACCCAG(+ATATAT)GTACCTGGGTAAATGTTTAACACAAGATGGA NGG 10 8 17 8 24 0 100 5 10
    240606235622_0z5w AGCAAGGCCCTGTGTTCTTGACATCTGAGAGG(+ATATAT)GATCCTACATATCAAGAGGGTCCGGGCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606235620_xqxa AGACAAGGAGCCCGGACCCTCTTGATATGTAG(+ATATAT)GATCCCTCTCAGATGTCAAGAACACAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240606235619_fga5 AGGTGATCCAAATGCCCTTTTGGCCCCTGCGG(+ATATAT)GTACCACATGTATGTGGTATGTGGATCTACA NGG 10 8 17 8 24 0 100 5 10