Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606235724_4mgk TAGAAATGGAACACAATGATGCAATGTATCTG(+ATATAT)GATCCCGTATGATTTTGTTTCGCTTATTAAC NGG 10 8 17 8 24 0 100 5 10
    240606235722_evca ACCACATGGTGGCTCACAACCATCTGTAATGG(+ATATAT)GATCCGATGCCCTCTTCTGGTGTGTCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606235721_nr6d GCTGTCTTCTGACACACCAGAAGAGGGCATCG(+ATATAT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606235719_hts6 CCCATAGTAATGACCCTTAAAGTTGACCTCAG(+ATATAT)GATCCACATACCAAGAAAAGAAATTGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606235717_b6gu CTCTAGCTCTCAATTTCTTTTCTTGGTATGTG(+ATATAT)GATCCTGAGGTCAACTTTAAGGGTCATTACT NGG 10 8 17 8 24 0 100 5 10
    240606235716_mqck ATTTTAAAATTAAGGCAGAGAGCACGAAGGAG(+ATATAT)GTACCCAGGTCAGCCGACGGCCTCCACACAC NGG 10 8 17 8 24 0 100 5 10
    240606235713_gsww TGCTTGTGTGTGGAGGCCGTCGGCTGACCTGG(+ATATAT)GTACCTCCTTCGTGCTCTCTGCCTTAATTTT NGG 10 8 17 8 24 0 100 5 10
    240606235712_5qpu TGCTCTGCCTCTCTGCTCAGGAACTAAGGATG(+ATATAT)GTACCCTTTATCCAAAGCAGGAAGTTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240606235711_5j4o GTTTCCTCACAACTTCCTGCTTTGGATAAAGG(+ATATAT)GTACCATCCTTAGTTCCTGAGCAGAGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240606235709_rh0k CAAAGGTCTAGAAAGGAAGGTTGCTTGGGCTG(+ATATAT)GATCCCTTCCCCTCCTTTTCTCCGATTAAAT NGG 10 8 17 8 24 0 100 5 10