Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606235617_iy6e CCACATGTAGATCCACATACCACATACATGTG(+ATATAT)GTACCCGCAGGGGCCAAAAGGGCATTTGGAT NGG 10 8 17 8 24 0 100 5 10
    240606235616_ow4p TCTCAATTAGTACTTATAGGGATGGGATTGCG(+ATATAT)GTACCACGGATACTGAAGCCCTCCCTGACTC NGG 10 8 17 8 24 0 100 5 10
    240606235614_u9sf GTCTAGAGTCAGGGAGGGCTTCAGTATCCGTG(+ATATAT)GTACCGCAATCCCATCCCTATAAGTACTAAT NGG 10 8 17 8 24 0 100 5 10
    240606235612_rgyk AACTAAACTGTACAGTAAGTTCTGCAGGGGTG(+ATATAT)GATCCCAATGTTTCTACCTTTATTTACAAAT NGG 10 8 17 8 24 0 100 5 10
    240606235610_y8tw GTGATATTTGTAAATAAAGGTAGAAACATTGG(+ATATAT)GATCCACCCCTGCAGAACTTACTGTACAGTT NGG 10 8 17 8 24 0 100 5 10
    240606235609_ohuk AACTAAACTGTACAGTAAGTTCTGCAGGGGTG(+ATATAT)GATCCCAATGTTTCTACCTTTATTTACAAAT NGG 10 8 17 8 24 0 100 5 10
    240606235607_j52p GTGATATTTGTAAATAAAGGTAGAAACATTGG(+ATATAT)GATCCACCCCTGCAGAACTTACTGTACAGTT NGG 10 8 17 8 24 0 100 5 10
    240606235605_ptif TTTTTTCTTCCATCCCAAGTAAAAGAGGGTTG(+ATATAT)GATCCTGTGTGTTCCTAGCAACAGCACCATT NGG 10 8 17 8 24 0 100 5 10
    240606235604_9t14 TGAGCAATGGTGCTGTTGCTAGGAACACACAG(+ATATAT)GATCCAACCCTCTTTTACTTGGGATGGAAGA NGG 10 8 17 8 24 0 100 5 10
    240606235603_9fb6 TAACTCTCTCATGTCTGAGCAGTTCTCTTTCG(+ATATAT)GATCCACTCTGCCTCGCTGCTGAGTCAGAGA NGG 10 8 17 8 24 0 100 5 10