Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607000226_o3gy AAGAGCTTTTTGACTGTCAGAGGAAATAAATG(+ATATAT)GTACCTAAAGTCCTTTTCTGTTTTCTCCATT NGG 10 8 17 8 24 0 100 5 10
    240607000225_lk3o ATAGTTAACCACATATCTAGAAATACCAGCAG(+ATATAT)GATCCTGGAGGGAGGGGGGAGGGGGAGGGGA NGG 10 8 17 8 24 0 100 5 10
    240607000223_55z6 CTCCTTCCCCTCCCCCTCCCCCCTCCCTCCAG(+ATATAT)GATCCTGCTGGTATTTCTAGATATGTGGTTA NGG 10 8 17 8 24 0 100 5 10
    240607000222_imyj TAAACAACTAATGTGGAAGGCTGATGCAGGAG(+ATATAT)GATCCATAGGAAACAGAAACAGCCCAAATGC NGG 10 8 17 8 24 0 100 5 10
    240607000220_xw3d GGGGGGCATTTGGGCTGTTTCTGTTTCCTATG(+ATATAT)GATCCTCCTGCATCAGCCTTCCACATTAGTT NGG 10 8 17 8 24 0 100 5 10
    240607000218_cq8s CCACCTGGATCTCAGAACTTAATTCAAGTGGG(+ATATAT)GTACCATATGTGATGGGTGTCTGTGATGGAG NGG 10 8 17 8 24 0 100 5 10
    240607000217_zmw6 CGTTTCTCCATCACAGACACCCATCACATATG(+ATATAT)GTACCCCACTTGAATTAAGTTCTGAGATCCA NGG 10 8 17 8 24 0 100 5 10
    240607000215_rd80 TAGGAGAAGTCATAATTAAATCCTGCAGTGGG(+ATATAT)GTACCAGTGACCCCAGTCACGTTGGACTAAG NGG 10 8 17 8 24 0 100 5 10
    240607000214_96y6 AGCTTCTTAGTCCAACGTGACTGGGGTCACTG(+ATATAT)GTACCCCACTGCAGGATTTAATTATGACTTC NGG 10 8 17 8 24 0 100 5 10
    240607000210_dogh CACTTAATTCTCTGACCGGGCCAGCATCATGG(+ATATAT)GTACCTGCTCCCCTGTGTACCCCATCCTTAA NGG 10 8 17 8 24 0 100 5 10