Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607000459_8z27 GCTATGGAGGAGAAACCTGGGAGCCTCTCTGG(+ATATAT)GATCCCTGTGTGAAGGAGCCTGAACTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240607000457_mdgc TGATGTAGAGTAGTTTATTCTGGTGTTGTGTG(+ATATAT)GATCCACTCAGTCCACAATCTAAAGACAGCA NGG 10 8 17 8 24 0 100 5 10
    240607000456_lf23 CTCAATGCTGTCTTTAGATTGTGGACTGAGTG(+ATATAT)GATCCACACAACACCAGAATAAACTACTCTA NGG 10 8 17 8 24 0 100 5 10
    240607000455_vfxo GCCTACAACATAACTAACTGACCCTCTTAGAG(+ATATAT)GATCCCAGTAACACCACATATAAAGAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240607000453_vq3s AACCGTCTTTTCTTTATATGTGGTGTTACTGG(+ATATAT)GATCCTCTAAGAGGGTCAGTTAGTTATGTTG NGG 10 8 17 8 24 0 100 5 10
    240607000452_bl1z TGGGAGGATCATTAGTTCAAGGCCAAACTAGG(+ATATAT)GTACCTTGTGATAACCTTTCATAAATATAAG NGG 10 8 17 8 24 0 100 5 10
    240607000451_wl8o AATGTCTTATATTTATGAAAGGTTATCACAAG(+ATATAT)GTACCCTAGTTTGGCCTTGAACTAATGATCC NGG 10 8 17 8 24 0 100 5 10
    240607000449_y6xo TCAGGCCCAGCCAAAGCTACCAGAGCTCTAAG(+ATATAT)GTACCTGGGGACCAATGGAATTTACACCTAT NGG 10 8 17 8 24 0 100 5 10
    240607000446_pryx TTACCATAGGTGTAAATTCCATTGGTCCCCAG(+ATATAT)GTACCTTAGAGCTCTGGTAGCTTTGGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607000445_o8vu GAGAAACCAAAATGCTGGTGACCCTTAGAGAG(+ATATAT)GATCCAGTCACCTTCCTTTAAGTCCTTCTCC NGG 10 8 17 8 24 0 100 5 10