Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607000603_lz2v TAGGCAGTCCTGAAACAAAGTGATAGGCACTG(+ATATAT)GTACCAGGAATGTTGCCTCAGCCAGAGAACT NGG 10 8 17 8 24 0 100 5 10
    240607000601_9sr1 GTGTGTGTGTGTGTGTGTGTGTGTACTCTAGG(+ATATAT)GTACCTCAGTATGTCCTGAAAGTTCAACAGA NGG 10 8 17 8 24 0 100 5 10
    240607000600_lkic AAAACTCTGTTGAACTTTCAGGACATACTGAG(+ATATAT)GTACCCTAGAGTACACACACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240607000559_yobx GAAAAGCACCATGGGAAATCCCTTGGCTTCTG(+ATATAT)GTACCAGCATGGTAATTGCAAGGGAAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240607000557_jv4g TCTGAATTTCTTCCCTTGCAATTACCATGCTG(+ATATAT)GTACCAGAAGCCAAGGGATTTCCCATGGTGC NGG 10 8 17 8 24 0 100 5 10
    240607000556_8h0e AGAACTTGGGTCTGCTTTCTACAGTCATTATG(+ATATAT)GTACCAAAACCATATGATTCTTTGTACAGTT NGG 10 8 17 8 24 0 100 5 10
    240607000555_qwju GGATAAACTGTACAAAGAATCATATGGTTTTG(+ATATAT)GTACCATAATGACTGTAGAAAGCAGACCCAA NGG 10 8 17 8 24 0 100 5 10
    240607000554_tvps AGCTTGTTTCTGAGAAAACCAAGTTCTAAGTG(+ATATAT)GTACCCAAAGGTGTTGCCAGTCCTTCCTATG NGG 10 8 17 8 24 0 100 5 10
    240607000552_g04l GGATGCATAGGAAGGACTGGCAACACCTTTGG(+ATATAT)GTACCACTTAGAACTTGGTTTTCTCAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240607000550_7w4e CTCCATGGTTAAGAACACTAGTTTTTGCTGAG(+ATATAT)GATCCCAGTTCAATTCCCAGTACCCACATGG NGG 10 8 17 8 24 0 100 5 10