Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606233642_ho2k TCCTTATAGCACTCAAGTGGCAGAGGCAGAAG(+ATATAT)GATCCCTAAGAATTTGAACCAGACTAGGTTA NGG 10 8 17 8 24 0 100 5 10
    240606233639_it6u CTATGTAACCTAGTCTGGTTCAAATTCTTAGG(+ATATAT)GATCCTTCTGCCTCTGCCACTTGAGTGCTAT NGG 10 8 17 8 24 0 100 5 10
    240606233637_uxim AAGTCCTCACAGAGGACACAGCTCAGGATCGG(+ATATAT)GATCCCACACCTGTAAAAAGTAAAGAACAAG NGG 10 8 17 8 24 0 100 5 10
    240606233635_p96l AACCACTTGTTCTTTACTTTTTACAGGTGTGG(+ATATAT)GATCCCGATCCTGAGCTGTGTCCTCTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240606233634_usg7 TGACACCTGATACCAGCAGGAGGTATACTTGG(+ATATAT)GATCCAATATGAATCAGTGGATTGCTCTGGT NGG 10 8 17 8 24 0 100 5 10
    240606233632_9mz4 CTAGAACCAGAGCAATCCACTGATTCATATTG(+ATATAT)GATCCCAAGTATACCTCCTGCTGGTATCAGG NGG 10 8 17 8 24 0 100 5 10
    240606233629_kbu3 AACTGCAGACTGGAGAGACCTGCACTTCTGGG(+ATATAT)GATCCTGCACCGACCTACAGCTTCCTTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606233627_dod7 AATTTCTTCAAGGAAGCTGTAGGTCGGTGCAG(+ATATAT)GATCCCCAGAAGTGCAGGTCTCTCCAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240606233625_s8l4 AGACTCTTCCAGGGGAGGGGTGCCAGGATGGG(+ATATAT)GTACCCCTGGTCACTCACCAGGATCACTGTA NGG 10 8 17 8 24 0 100 5 10
    240606233622_cnnn AGGGTTACAGTGATCCTGGTGAGTGACCAGGG(+ATATAT)GTACCCCATCCTGGCACCCCTCCCCTGGAAG NGG 10 8 17 8 24 0 100 5 10