Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606233523_b4ex GGCTGGAACATTCACTTTGTACAGTACCATTG(+ATATAT)GTACCAGCAAGAAATATCCACAGGAATAAAT NGG 10 8 17 8 24 0 100 5 10
    240606233522_0vuu ATTTCATTTATTCCTGTGGATATTTCTTGCTG(+ATATAT)GTACCAATGGTACTGTACAAAGTGAATGTTC NGG 10 8 17 8 24 0 100 5 10
    240606233520_7ezn TATCTTCAGGAGATATGTTACTGCACTTGCAG(+ATATAT)GTACCTTAATTCACTTGAACATTTACAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606233519_9xw6 AGTGTCCTTTGTAAATGTTCAAGTGAATTAAG(+ATATAT)GTACCTGCAAGTGCAGTAACATATCTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240606233517_q8j0 ATAAACAACAAAGCTAGTCATTTTGTCCTATG(+ATATAT)GATCCTGTCCTCACATTAAACACTGTCCAGT NGG 10 8 17 8 24 0 100 5 10
    240606233515_tw6k CCGCCACTGGACAGTGTTTAATGTGAGGACAG(+ATATAT)GATCCATAGGACAAAATGACTAGCTTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240606233513_uxsg ACCCTTTTCTTAGGGCACACAAATTAATCTAG(+ATATAT)GTACCTTGTTGTGTACAATTGAGAACACCTT NGG 10 8 17 8 24 0 100 5 10
    240606233512_flf9 AAGTCAAGGTGTTCTCAATTGTACACAACAAG(+ATATAT)GTACCTAGATTAATTTGTGTGCCCTAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606233510_rouc AAAGCAAATGGCAGAGCCTTTTGATATCAGAG(+ATATAT)GATCCTCTTACACTTCTTCCCCCCTCCATTG NGG 10 8 17 8 24 0 100 5 10
    240606233508_va5m CAGCACAATGGAGGGGGGAAGAAGTGTAAGAG(+ATATAT)GATCCTCTGATATCAAAAGGCTCTGCCATTT NGG 10 8 17 8 24 0 100 5 10