Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606233507_j986 TACATAGTTCAGAGTTGCTTCTGGGGGATCTG(+ATATAT)GTACCTTTAATATTTAAACTTTGAGCAATAT NGG 10 8 17 8 24 0 100 5 10
    240606233505_pgrn GCAGAATATTGCTCAAAGTTTAAATATTAAAG(+ATATAT)GTACCAGATCCCCCAGAAGCAACTCTGAACT NGG 10 8 17 8 24 0 100 5 10
    240606233504_9nvf CCCTTGCAGATAATGTTATGCCCTTGAGATAG(+ATATAT)GTACCCAATGCAAATTAAATGAATGTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606233502_wcdx TCAGTATTTTACATTCATTTAATTTGCATTGG(+ATATAT)GTACCTATCTCAAGGGCATAACATTATCTGC NGG 10 8 17 8 24 0 100 5 10
    240606233500_14v0 GTACATAACGTCCTTCAGTCATATTCATCCAG(+ATATAT)GTACCCTCCTTTGTCCCAGTCCCACCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240606233457_vn9j GGATAAGAGGGGTGGGACTGGGACAAAGGAGG(+ATATAT)GTACCTGGATGAATATGACTGAAGGACGTTA NGG 10 8 17 8 24 0 100 5 10
    240606233455_89i9 AGTGGCTCGTTAAGCTTTCCTTTTACCAGTTG(+ATATAT)GTACCAACTTTTAGCATTTCATAAAGTTTGT NGG 10 8 17 8 24 0 100 5 10
    240606233453_dt4p ATTCTACAAACTTTATGAAATGCTAAAAGTTG(+ATATAT)GTACCAACTGGTAAAAGGAAAGCTTAACGAG NGG 10 8 17 8 24 0 100 5 10
    240606233451_ifc0 GTCCAGATGTGCGGCAGCCGTGGAGCTTCAGG(+ATATAT)GTACCCTGGAGCTCACAGGCTCTCTGGGAGT NGG 10 8 17 8 24 0 100 5 10
    240606233449_jfb8 GGCAGACTCCCAGAGAGCCTGTGAGCTCCAGG(+ATATAT)GTACCCTGAAGCTCCACGGCTGCCGCACATC NGG 10 8 17 8 24 0 100 5 10