Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606233350_8n1p AACATTCCAGGCCGTTCGGAAGGCATCACTGG(+ATATAT)GTACCAGACTTGAAACCAGTCTTCAGTAACA NGG 10 8 17 8 24 0 100 5 10
    240606233348_nq4s ACACTTGTTACTGAAGACTGGTTTCAAGTCTG(+ATATAT)GTACCCAGTGATGCCTTCCGAACGGCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606233347_0ly1 CCGTGGGATTATAGGAAAACCAGGGCCTCTTG(+ATATAT)GTACCCTGTCCTCAGAGTGTGTGACAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606233345_201q TTACAGTGGCTGTCACACACTCTGAGGACAGG(+ATATAT)GTACCAAGAGGCCCTGGTTTTCCTATAATCC NGG 10 8 17 8 24 0 100 5 10
    240606233343_njl4 GCACCTCCGAAGCTCTTAGTAATGTTGTGCTG(+ATATAT)GATCCTGTAAGTTCGCTCTGCAGTCACTCGC NGG 10 8 17 8 24 0 100 5 10
    240606233342_l6li GGTTTGCGAGTGACTGCAGAGCGAACTTACAG(+ATATAT)GATCCAGCACAACATTACTAAGAGCTTCGGA NGG 10 8 17 8 24 0 100 5 10
    240606233340_eso1 TGATGCTCCACAAACTGTCTCATTAATCCATG(+ATATAT)GATCCTAAAACAAATTCTCTTTGTGTATTGT NGG 10 8 17 8 24 0 100 5 10
    240606233338_g454 CACACACAATACACAAAGAGAATTTGTTTTAG(+ATATAT)GATCCATGGATTAATGAGACAGTTTGTGGAG NGG 10 8 17 8 24 0 100 5 10
    240606233336_q9bb TCTTCCTTTTGAAGCTAGGCTGTGCTGTGTGG(+ATATAT)GATCCAGTGGTCTGTTCTTTACTGGAGGTAT NGG 10 8 17 8 24 0 100 5 10
    240606233334_l7vl AACACATACCTCCAGTAAAGAACAGACCACTG(+ATATAT)GATCCCACACAGCACAGCCTAGCTTCAAAAG NGG 10 8 17 8 24 0 100 5 10