Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606233309_q93p CAGGGCCAAGAAGTAGGAGTGGGTGAGTAGGG(+ATATAT)GATCCGGGTGGAGGTATAGGGGACTTTCAGG NGG 10 8 17 8 24 0 100 5 10
    240606233307_cpxz GCTATCCTGAAAGTCCCCTATACCTCCACCCG(+ATATAT)GATCCCCTACTCACCCACTCCTACTTCTTGG NGG 10 8 17 8 24 0 100 5 10
    240606233305_bsp8 GAGGTTGTCTCTGGCTCTGTTGTCTGCTGTTG(+ATATAT)GATCCCTTTCTCCTACCTGGACTGTCTGGTT NGG 10 8 17 8 24 0 100 5 10
    240606233303_lhj3 GGCCCAACCAGACAGTCCAGGTAGGAGAAAGG(+ATATAT)GATCCAACAGCAGACAACAGAGCCAGAGACA NGG 10 8 17 8 24 0 100 5 10
    240606233302_e8sl GGAAGAAGAACCTTTGTAACCTGTTAATGGAG(+ATATAT)GATCCTTATAAAACAGAACAACTCTGTATAA NGG 10 8 17 8 24 0 100 5 10
    240606233300_l6bt CCGGTTTATACAGAGTTGTTCTGTTTTATAAG(+ATATAT)GATCCTCCATTAACAGGTTACAAAGGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240606233259_ac99 TGGGACACGCCCCCCCCCCCCCACTTGAGCTG(+ATATAT)GATCCCACTTTGTGCCTTTCTCTGGACCTTC NGG 10 8 17 8 24 0 100 5 10
    240606233257_a16w GAAAAGAAGGTCCAGAGAAAGGCACAAAGTGG(+ATATAT)GATCCAGCTCAAGTGGGGGGGGGGGGGCGTG NGG 10 8 17 8 24 0 100 5 10
    240606233255_lcj5 ATGGTAATAAATAAATAAGATTTCTTTTAATG(+ATATAT)GATCCAGACTTACCTGAGGACAACTGTAGGG NGG 10 8 17 8 24 0 100 5 10
    240606233253_vkps ATTAACCCTACAGTTGTCCTCAGGTAAGTCTG(+ATATAT)GATCCATTAAAAGAAATCTTATTTATTTATT NGG 10 8 17 8 24 0 100 5 10