Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606233230_s4dq CATCCCTTTGTCACTAAAGGGAACAACATGGG(+ATATAT)GTACCAGGCAGTCAAAAGCTGTAAAAACCCC NGG 10 8 17 8 24 0 100 5 10
    240606233228_v3i2 AAAATGGGGTTTTTACAGCTTTTGACTGCCTG(+ATATAT)GTACCCCATGTTGTTCCCTTTAGTGACAAAG NGG 10 8 17 8 24 0 100 5 10
    240606233227_ufs2 TCTCTAGCCAGCCCTGCTGCTCCTGTTGTCTG(+ATATAT)GTACCCAGGTGACTGAGGACATGACTGGTGG NGG 10 8 17 8 24 0 100 5 10
    240606233224_3zsc TCCTTCCACCAGTCATGTCCTCAGTCACCTGG(+ATATAT)GTACCAGACAACAGGAGCAGCAGGGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240606233222_4gqg GTGCACAGTTCTGTGAGGCTTTGGGGCAAGGG(+ATATAT)GTACCAAGAGCCCAAGAGAGCCTTTCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240606233221_hipe TTTAGAACAAGAAAGGCTCTCTTGGGCTCTTG(+ATATAT)GTACCCCTTGCCCCAAAGCCTCACAGAACTG NGG 10 8 17 8 24 0 100 5 10
    240606233219_808y TAGGGAGGGATGTCAAGGCCAAGAGTCATACG(+ATATAT)GTACCCAGTAACTGCTGGTGTAGCTCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240606233217_cd0y TGCCTCCCAAGAGCTACACCAGCAGTTACTGG(+ATATAT)GTACCGTATGACTCTTGGCCTTGACATCCCT NGG 10 8 17 8 24 0 100 5 10
    240606233215_fb4a CCAACAGGGAGGGACCAAGGGCAGCCACCAGG(+ATATAT)GATCCTATCTCACCTCCGATGCCTGCTGTGT NGG 10 8 17 8 24 0 100 5 10
    240606233213_usa2 CCTGGACACAGCAGGCATCGGAGGTGAGATAG(+ATATAT)GATCCCTGGTGGCTGCCCTTGGTCCCTCCCT NGG 10 8 17 8 24 0 100 5 10