Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606233151_zqi0 TGGAGGCCCCAGGGCCAGAGTCCAAAGCCAGG(+ATATAT)GTACCAGCTGCAGGAGATGGAGAAGGGGCCA NGG 10 8 17 8 24 0 100 5 10
    240606233149_xfoe CTCCCTGGCCCCTTCTCCATCTCCTGCAGCTG(+ATATAT)GTACCCTGGCTTTGGACTCTGGCCCTGGGGC NGG 10 8 17 8 24 0 100 5 10
    240606233148_h59h ACCGTGTGATAAGGTTGTGTGCTGTGTGATCG(+ATATAT)GTACCCAGGGAGCGGGGCGGTGTTAATGTGC NGG 10 8 17 8 24 0 100 5 10
    240606233144_4l5k ATTTGGCACATTAACACCGCCCCGCTCCCTGG(+ATATAT)GTACCGATCACACAGCACACAACCTTATCAC NGG 10 8 17 8 24 0 100 5 10
    240606233142_wudw TTGCTGATGCTCAGTGGTCCAGGCTACGCTGG(+ATATAT)GATCCGTGATGGGGATATCCCAGAAGACACC NGG 10 8 17 8 24 0 100 5 10
    240606233141_abc3 GGAATGGTGTCTTCTGGGATATCCCCATCACG(+ATATAT)GATCCCAGCGTAGCCTGGACCACTGAGCATC NGG 10 8 17 8 24 0 100 5 10
    240606233139_ui7p ACAGCAATCCATGGCTTTCCAGAAGAGGTGTG(+ATATAT)GATCCAAAAGTGCCACCTCTATCTTAGTCAC NGG 10 8 17 8 24 0 100 5 10
    240606233138_sous TGCTGGTGACTAAGATAGAGGTGGCACTTTTG(+ATATAT)GATCCACACCTCTTCTGGAAAGCCATGGATT NGG 10 8 17 8 24 0 100 5 10
    240606233135_qj42 TCAAGTGCCAAAGCTGTCTCGTGAGCTTCCAG(+ATATAT)GATCCTGACAGGCCTGGAGGGAACTGCAAAG NGG 10 8 17 8 24 0 100 5 10
    240606233134_hyyw TGGCCCTTTGCAGTTCCCTCCAGGCCTGTCAG(+ATATAT)GATCCTGGAAGCTCACGAGACAGCTTTGGCA NGG 10 8 17 8 24 0 100 5 10