Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606233054_bckg CACTGGAGGTGCAGGTGCCTGGGCCAGCCTTG(+ATATAT)GATCCATATCCAGTATTTGGCAGATTCATGC NGG 10 8 17 8 24 0 100 5 10
    240606233053_soq8 GGCAGGCATGAATCTGCCAAATACTGGATATG(+ATATAT)GATCCAAGGCTGGCCCAGGCACCTGCACCTC NGG 10 8 17 8 24 0 100 5 10
    240606233051_u0v9 TGCCCATCGCTAAAGCTAAGACCTGCAGTAAG(+ATATAT)GATCCCTCAGAGCCTCTTTTCAAAGCATGGT NGG 10 8 17 8 24 0 100 5 10
    240606233049_2jta GAGCAACCATGCTTTGAAAAGAGGCTCTGAGG(+ATATAT)GATCCTTACTGCAGGTCTTAGCTTTAGCGAT NGG 10 8 17 8 24 0 100 5 10
    240606233047_6yka AAAAAGCTACAAACATTTCCAACAGACAAATG(+ATATAT)GATCCTAGCTGCCCCATGCATGGTTCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240606233046_4aef CATGCTCCCAGAACCATGCATGGGGCAGCTAG(+ATATAT)GATCCATTTGTCTGTTGGAAATGTTTGTAGC NGG 10 8 17 8 24 0 100 5 10
    240606233044_773d TAGGGGCTGGAGCTGAGCTGCCTTCTGTCCTG(+ATATAT)GTACCTGGAGGGAACGAGGATGTTAAGTGAT NGG 10 8 17 8 24 0 100 5 10
    240606233042_uqld AGTCAATCACTTAACATCCTCGTTCCCTCCAG(+ATATAT)GTACCAGGACAGAAGGCAGCTCAGCTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606233040_85s0 CTGCCCACTGTGCCTCAGCTGTGTGGAGCCTG(+ATATAT)GTACCAGAGTCAGGCCCCAGAGAAGGGAGGA NGG 10 8 17 8 24 0 100 5 10
    240606233038_yjre TTTTGTCCTCCCTTCTCTGGGGCCTGACTCTG(+ATATAT)GTACCAGGCTCCACACAGCTGAGGCACAGTG NGG 10 8 17 8 24 0 100 5 10