Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606232943_jo8n TCCCTTCAACTTTCCCTAGTCTACATCTGCAG(+ATATAT)GTACCAATAAGACCATCTACATAACCTGCAA NGG 10 8 17 8 24 0 100 5 10
    240606232941_7nob GTTTCTTGCAGGTTATGTAGATGGTCTTATTG(+ATATAT)GTACCTGCAGATGTAGACTAGGGAAAGTTGA NGG 10 8 17 8 24 0 100 5 10
    240606232940_bp2y TCCTCCACAAATAAACGTAATTAAATCTAAAG(+ATATAT)GTACCCTGAGATTTCTGTATAGGAAACAACT NGG 10 8 17 8 24 0 100 5 10
    240606232938_g72v AAAACAGTTGTTTCCTATACAGAAATCTCAGG(+ATATAT)GTACCTTTAGATTTAATTACGTTTATTTGTG NGG 10 8 17 8 24 0 100 5 10
    240606232936_g6ag AGGTTTCTAGAAACACGCAGACACTGAGAATG(+ATATAT)GTACCCAGGGTCCTGCTCTTTACCACAGGTT NGG 10 8 17 8 24 0 100 5 10
    240606232933_lfdv GCATGAACCTGTGGTAAAGAGCAGGACCCTGG(+ATATAT)GTACCATTCTCAGTGTCTGCGTGTTTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240606232932_javn AGAGGTTAGATGTTCTTGGCCTGCAGGGACAG(+ATATAT)GTACCTATAGTGTTGGCATAGTCAGGTTTCC NGG 10 8 17 8 24 0 100 5 10
    240606232931_olv8 TGCTGGGAAACCTGACTATGCCAACACTATAG(+ATATAT)GTACCTGTCCCTGCAGGCCAAGAACATCTAA NGG 10 8 17 8 24 0 100 5 10
    240606232929_cxcb TGCCGTCGGGCTGGATCCTAGGTGGACACTTG(+ATATAT)GTACCTCTCTGTTGCTGTTTCTTGGAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240606232928_ilid AGCTGGGCTCTCCAAGAAACAGCAACAGAGAG(+ATATAT)GTACCAAGTGTCCACCTAGGATCCAGCCCGA NGG 10 8 17 8 24 0 100 5 10