Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606232908_2ihz GAGTGCCATCAGAGGCGTTATACCACAGTCTG(+ATATAT)GATCCAGGCCAGAAGGGATGTGTGGGGGATG NGG 10 8 17 8 24 0 100 5 10
    240606232905_iqo0 CTCTTCATCCCCCACACATCCCTTCTGGCCTG(+ATATAT)GATCCAGACTGTGGTATAACGCCTCTGATGG NGG 10 8 17 8 24 0 100 5 10
    240606232903_e2yh CCTGAACCCATGGAACTTCATGACTTTTGCTG(+ATATAT)GATCCATTTCCCTTAGTGGCTTCCAGAGCCC NGG 10 8 17 8 24 0 100 5 10
    240606232902_xar9 AACTGGGGCTCTGGAAGCCACTAAGGGAAATG(+ATATAT)GATCCAGCAAAAGTCATGAAGTTCCATGGGT NGG 10 8 17 8 24 0 100 5 10
    240606232900_owji CTGGGTGTGGCCTCAGCTCCTGTGCTTGTGTG(+ATATAT)GATCCCTAAAGGGTGTGCTAAGGAGCAATTG NGG 10 8 17 8 24 0 100 5 10
    240606232858_pqr5 CAGTGCAATTGCTCCTTAGCACACCCTTTAGG(+ATATAT)GATCCACACAAGCACAGGAGCTGAGGCCACA NGG 10 8 17 8 24 0 100 5 10
    240606232856_fglx CAGTTTGCGGACAAACCTGATGACCTGAGTTG(+ATATAT)GATCCCTGACCATAAGGTAGAAGAGACCTGA NGG 10 8 17 8 24 0 100 5 10
    240606232854_4bck AGGAATCAGGTCTCTTCTACCTTATGGTCAGG(+ATATAT)GATCCAACTCAGGTCATCAGGTTTGTCCGCA NGG 10 8 17 8 24 0 100 5 10
    240606232852_w1hr TGGGGCGCACATCCCACCTCAGTGTTCTAAAG(+ATATAT)GATCCAGGGCCACTTTGTCTTCAAGTGTCAT NGG 10 8 17 8 24 0 100 5 10
    240606232849_fw1r CACAAATGACACTTGAAGACAAAGTGGCCCTG(+ATATAT)GATCCTTTAGAACACTGAGGTGGGATGTGCG NGG 10 8 17 8 24 0 100 5 10