Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606232744_66f5 TCCGCACATGGAGAACAGGAGCCGGGCCCTGG(+ATATAT)GATCCTTCTGTTCTGTTAAACAGAACAGAAC NGG 10 8 17 8 24 0 100 5 10
    240606232742_ogs1 AGGCTGTTCTGTTCTGTTTAACAGAACAGAAG(+ATATAT)GATCCCAGGGCCCGGCTCCTGTTCTCCATGT NGG 10 8 17 8 24 0 100 5 10
    240606232740_h7jl TAGACTCTGCCCTCGTCTTGCTCTCCAGGTTG(+ATATAT)GTACCTCAGCCCCTCTATTGTGAACTTAGAT NGG 10 8 17 8 24 0 100 5 10
    240606232739_540o TCCCTATCTAAGTTCACAATAGAGGGGCTGAG(+ATATAT)GTACCAACCTGGAGAGCAAGACGAGGGCAGA NGG 10 8 17 8 24 0 100 5 10
    240606232735_q22c CAGCCCCCAGCCCGGGAATGTCTGTTGCAGGG(+ATATAT)GATCCTCTGTCTCCAGCCCTGGCAAGGTCCC NGG 10 8 17 8 24 0 100 5 10
    240606232733_k8qf TGGCAGGGACCTTGCCAGGGCTGGAGACAGAG(+ATATAT)GATCCCCTGCAACAGACATTCCCGGGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606232731_tgy9 GACCTTCTGCACTCCATCCATAATAGCCTTTG(+ATATAT)GTACCTTCCAGAGGAGTCCTTCCCGAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240606232729_0u16 TTGCCAGCTCTCGGGAAGGACTCCTCTGGAAG(+ATATAT)GTACCAAAGGCTATTATGGATGGAGTGCAGA NGG 10 8 17 8 24 0 100 5 10
    240606232727_5hta AGAAAGCACCAGGTCAGGCAGGGCCTGAGGTG(+ATATAT)GATCCGAGCTGGGTCCAGGGCTCGAATCTCA NGG 10 8 17 8 24 0 100 5 10
    240606232725_z4hz TGGTGTGAGATTCGAGCCCTGGACCCAGCTCG(+ATATAT)GATCCACCTCAGGCCCTGCCTGACCTGGTGC NGG 10 8 17 8 24 0 100 5 10