Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606232703_pivb TATACTTGGGGACAGTTACAAGGGAGTCACAG(+ATATAT)GTACCCTGTCCATGTCTGCGGCTGTGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240606232700_4und GCAGGCTGCCCACAGCCGCAGACATGGACAGG(+ATATAT)GTACCTGTGACTCCCTTGTAACTGTCCCCAA NGG 10 8 17 8 24 0 100 5 10
    240606232659_5g0k CAAGTCAGAATTCCTGGTAGGGGCGGTTTCAG(+ATATAT)GATCCCACAGAAATGAAGGGACACAGTCTTC NGG 10 8 17 8 24 0 100 5 10
    240606232657_6tig TGGGAGAAGACTGTGTCCCTTCATTTCTGTGG(+ATATAT)GATCCTGAAACCGCCCCTACCAGGAATTCTG NGG 10 8 17 8 24 0 100 5 10
    240606232655_dzmn CATCCAGGCCCCCAATCTTTTATATTGAAGGG(+ATATAT)GATCCAACTTCCAGGATGAAAGGCAGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606232653_w6mo ACCAGGCTCTCTGCCTTTCATCCTGGAAGTTG(+ATATAT)GATCCCCTTCAATATAAAAGATTGGGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240606232651_tm1r GCTGAGCAAGAAAGGCTGTCTGAGCCATGGTG(+ATATAT)GATCCCCAGGGAGGGAATGTCCCCTCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240606232649_zv77 GGGCAGGGGGGAGGGGACATTCCCTCCCTGGG(+ATATAT)GATCCACCATGGCTCAGACAGCCTTTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240606232647_xkxt GCCTACTTCTGGATCACCCTCATCCAGTGCTG(+ATATAT)GTACCCCACAGCACTAAATCCAGGAACCCTG NGG 10 8 17 8 24 0 100 5 10
    240606232644_43ca AAGCCCAGGGTTCCTGGATTTAGTGCTGTGGG(+ATATAT)GTACCAGCACTGGATGAGGGTGATCCAGAAG NGG 10 8 17 8 24 0 100 5 10