Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606232539_dj0x GAGCTTCTTTGGTATCACCTGTCTATGTGCAG(+ATATAT)GATCCTTCCAAAATGGAAGTAAACTACGCAG NGG 10 8 17 8 24 0 100 5 10
    240606232537_mygh GGGTGCTGCGTAGTTTACTTCCATTTTGGAAG(+ATATAT)GATCCTGCACATAGACAGGTGATACCAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606232534_6osd TGTGTCTGGAACTCACCAAGGACACTAGCCCG(+ATATAT)GATCCCTAGACAGAAGCTATGGACCCCATAA NGG 10 8 17 8 24 0 100 5 10
    240606232532_mi3g GGGATTTATGGGGTCCATAGCTTCTGTCTAGG(+ATATAT)GATCCGGGCTAGTGTCCTTGGTGAGTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240606232530_shri TCTGTTCAATAGAGGCCACTTCCTTCCCAAGG(+ATATAT)GATCCAAAACCCAAAACCTGCGGCTTCCACT NGG 10 8 17 8 24 0 100 5 10
    240606232529_e99h GTTTCAGTGGAAGCCGCAGGTTTTGGGTTTTG(+ATATAT)GATCCCTTGGGAAGGAAGTGGCCTCTATTGA NGG 10 8 17 8 24 0 100 5 10
    240606232526_scyc AAGTCCATTTCCCAGCCATACGCAGCTGTGGG(+ATATAT)GATCCCAGGAGACACTTCAAGTGGAATGAAT NGG 10 8 17 8 24 0 100 5 10
    240606232524_tba8 TTGAGATTCATTCCACTTGAAGTGTCTCCTGG(+ATATAT)GATCCCCACAGCTGCGTATGGCTGGGAAATG NGG 10 8 17 8 24 0 100 5 10
    240606232522_b63d TGGAATGGCTGTCCTGTAGGCAGGTTGGGGTG(+ATATAT)GTACCCTGGGGCTTCTGGGAATGGGGCTATG NGG 10 8 17 8 24 0 100 5 10
    240606232518_b2tv AACACCATAGCCCCATTCCCAGAAGCCCCAGG(+ATATAT)GTACCACCCCAACCTGCCTACAGGACAGCCA NGG 10 8 17 8 24 0 100 5 10