Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606232427_ok50 TCTTGCAAGGGACTCTTCCCCCTGCCCCTCAG(+ATATAT)GATCCAAAAGGCCCAGGCTGAGGAAACTCAT NGG 10 8 17 8 24 0 100 5 10
    240606232425_n1iz CCTCCATGAGTTTCCTCAGCCTGGGCCTTTTG(+ATATAT)GATCCTGAGGGGCAGGGGGAAGAGTCCCTTG NGG 10 8 17 8 24 0 100 5 10
    240606232422_murb ACAACATAGTGGCTCACAACCATCTGTAATGG(+ATATAT)GATCCGATGCCCGCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606232420_7cve GCTGTCTTCAGACACACCAGAAGCGGGCATCG(+ATATAT)GATCCCATTACAGATGGTTGTGAGCCACTAT NGG 10 8 17 8 24 0 100 5 10
    240606232419_am5t GGCCCGGGGCTCCTATTCTTATGTGGCTGCAG(+ATATAT)GATCCTCTGGAAATGACTATGATTTAATGGC NGG 10 8 17 8 24 0 100 5 10
    240606232416_cdbs GCTGAGCCATTAAATCATAGTCATTTCCAGAG(+ATATAT)GATCCTGCAGCCACATAAGAATAGGAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240606232412_cxr7 TCTCAAGACTGCAGCCACGTAAACTTCCTCTG(+ATATAT)GATCCTAAGTTACTATATAAACGGATCTTTA NGG 10 8 17 8 24 0 100 5 10
    240606232411_4wtk GGTTTTAAAGATCCGTTTATATAGTAACTTAG(+ATATAT)GATCCAGAGGAAGTTTACGTGGCTGCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240606232409_8nmp CTATCTTTTCCTAAAGGCAGTTTCCCTGCTAG(+ATATAT)GATCCCTAAAGTGATTATTGAAGAAAATACA NGG 10 8 17 8 24 0 100 5 10
    240606232407_sibn GCTTCTGTATTTTCTTCAATAATCACTTTAGG(+ATATAT)GATCCTAGCAGGGAAACTGCCTTTAGGAAAA NGG 10 8 17 8 24 0 100 5 10