Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606232325_ym08 GCTGAGTGAGGCCACTCCCATTGAAGAGTGAG(+ATATAT)GATCCTGGAGCCCCAAAGGCCTTTGGGAATG NGG 10 8 17 8 24 0 100 5 10
    240606232323_exn9 TAGCTCATTCCCAAAGGCCTTTGGGGCTCCAG(+ATATAT)GATCCTCACTCTTCAATGGGAGTGGCCTCAC NGG 10 8 17 8 24 0 100 5 10
    240606232321_sop1 TCCCGTCATGTTTCTCCTTACTCATCCAATTG(+ATATAT)GTACCCACAACTGAAGAGCTCGTGTATTTGG NGG 10 8 17 8 24 0 100 5 10
    240606232319_ng99 GCCCGCCAAATACACGAGCTCTTCAGTTGTGG(+ATATAT)GTACCAATTGGATGAGTAAGGAGAAACATGA NGG 10 8 17 8 24 0 100 5 10
    240606232317_3imp CTCTTTTGTCTGTGGAGTCTGCTGTGTGGCAG(+ATATAT)GATCCCAGCCCTAGAGCTGCAGGGCTGCCCT NGG 10 8 17 8 24 0 100 5 10
    240606232316_6kah CCCACAGGGCAGCCCTGCAGCTCTAGGGCTGG(+ATATAT)GATCCTGCCACACAGCAGACTCCACAGACAA NGG 10 8 17 8 24 0 100 5 10
    240606232313_zm9h CTGGGTGGTGGTCTGTCTGGTTCCAGTGGGAG(+ATATAT)GTACCATCTCTTCTCCTTTGAGAAATGCCCC NGG 10 8 17 8 24 0 100 5 10
    240606232312_o62l CTGCAGGGGCATTTCTCAAAGGAGAAGAGATG(+ATATAT)GTACCTCCCACTGGAACCAGACAGACCACCA NGG 10 8 17 8 24 0 100 5 10
    240606232310_p16t TCTGGGGTAGCTTACCCAGTGCTAGGCACTGG(+ATATAT)GATCCTGAAAGTACCTTTCTTGGTGATCTGG NGG 10 8 17 8 24 0 100 5 10
    240606232308_hoyo CTGTTCCAGATCACCAAGAAAGGTACTTTCAG(+ATATAT)GATCCCAGTGCCTAGCACTGGGTAAGCTACC NGG 10 8 17 8 24 0 100 5 10